听力相关基因多态性与噪声性听力损失易感关联性

发布时间：2018-01-22 04:02

本文关键词： 噪声听力损失易感性单核苷酸多态性关联性　出处：《中国职业医学》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨听力相关基因中3'非翻译区单核苷酸多态性(SNP)与中国汉族人群噪声性听力损失(NIHL)的易感关联性。方法采用判断抽样法,选择广州市3家大型压缩机生产企业接触40 h等效声级为72~120 d B(A)的2 507名工人为研究对象,结合工作场所噪声强度测量结果和工人纯音测听结果,筛选易感组和耐受组人群各238人。获取2组人群周围血基因组DNA,采用Taq Man探针法化学荧光等位基因鉴别试验进行遗传特征分析。结果易感组人群左耳听阈加权值(MTWV)大于右耳(P0.01);易感组人群的左耳、右耳MTWV分别高于耐受组人群左耳和右耳MTWV(P0.01)。共筛选4个候选基因:囊泡相关膜蛋白1(VAMP1)、成纤维细胞生长因子1(FGF1)、钾离子内向整流通道蛋白J亚单位10号成员(KCNJ10)和肌球蛋白1C(MYO1C)。基因SNP位点检测结果显示,易感组人群携带FGF1 rs17217562位点AC和CC基因型均多于耐受组(P0.05),携带C等位基因多于耐受组(P0.05)。Logistic回归分析结果显示,校正年龄、噪声暴露水平、噪声作业工龄、性别、吸烟、饮酒,以及是否使用耳机、接触有机溶剂、重金属、高温和手传振动等混杂因素后,FGF1 rs17217562位点携带等位基因C的人群患NIHL易感风险增加(P0.05)。未发现VAMP1、KCNJ10和MYO1C的SNP与NIHL易感有关联。结论中国汉族人群的FGF1 rs17217562位点SNP可能与NIHL易感性有关。
[Abstract]:Objective to investigate the association between the single nucleotide polymorphisms (SNPs) of 3'untranslated region (SNPs) and noise-induced hearing loss (NIHL) in Chinese Han population. A total of 2 507 workers with an equivalent sound level of 722o120 dB were selected from 3 large compressor manufacturing enterprises in Guangzhou. Based on the results of workplace noise intensity measurement and pure tone audiometry, 238 people in susceptible group and 238 subjects in tolerance group were selected. The peripheral blood genomic DNA was obtained from two groups. The genetic characteristics were analyzed by Taq Man probe chemofluorescence allele identification test. Results in susceptible group, the weighted value of left ear hearing threshold was greater than that of right ear (P 0.01). The MTWV of left ear and right ear of susceptible group were higher than that of left ear and right ear of tolerance group respectively. Four candidate genes were screened: vesicular associated membrane protein 1 (VAMP1). Fibroblast growth factor (FGF1). Kalium inward rectifier channel protein J subunit 10 (KCNJ10) and myosin 1CU MYO1C0. The SNP loci of the gene were detected. The AC and CC genotypes of FGF1 rs17217562 locus were higher in susceptible group than in tolerant group (P0.05). The results of logistic regression analysis showed that the adjusted age, noise exposure level, noise working age, sex, smoking and drinking were higher than those of tolerance group. And whether to use headphones, exposure to organic solvents, heavy metals, high temperatures and hand-passed vibration after mixed factors. The population with allele C at FGF1 rs17217562 locus had an increased risk of NIHL. No VAMP1 was found. SNP of KCNJ10 and MYO1C is associated with susceptibility to NIHL. Conclusion SNP at FGF1 rs17217562 locus may be associated with NIHL susceptibility in Chinese Han population.
【作者单位】：佛山市职业病防治所;广州市职业病防治院职业环境与健康效应实验室;广州医科大学公共卫生学院;
【基金】：国家自然科学基金(81470146) 广州市民生科技重大项目(2014Y2-00067) 广东省“十二五”医学重点专科广州市医学重点学科建设项目广州市职业环境与健康效应实验室建设项目(2014SY000020) 广州市“121人才梯队工程”后备人才项目
【分类号】：R135.8
【正文快照】： 3.广州医科大学公共卫生学院,511436噪声性听力损失(Noise induced hearing loss,NIHL)是由于长期受噪声刺激而发生的一种缓慢的、进行性的听觉损伤[1-2],其损伤程度除了与噪声的性质、强度、频率、接触时间有关外,还与遗传易感性有关[3-4]。单核苷酸多态性(Single nucleotide

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