KCNJ11基因多态与糖尿病前期人群运动干预敏感性的相关性研究

发布时间：2018-01-23 19:06

本文关键词： 糖尿病前期 KCNJ基因基因多态性运动干预　出处：《中国糖尿病杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的研究钾离子通道蛋白KCNJ11基因区的3个标签单核苷酸多态性位点(SNP),及其组成的单体型与糖尿病前期人群运动干预前后糖脂代谢相关指标变化的关系,探讨其作为糖代谢异常人群对运动干预敏感的分子标记。方法对70名糖尿病前期人群进行为期12周的有氧耐力运动干预,测定其干预前后糖脂代谢相关生化指标。采用MALDI-TOF MS方法解析受试者基因组DNA多态位点基因型,分析干预前后相关指标的变化与单个位点及多个位点组成的单体型的相关性。结果 rs5219位点的CC基因型与HOMA-IR、TC、TG指标的下降、与HDL-C指标的升高相关,rs2285676位点的GG基因型与FPG、HbA1c、HOMA-IR、TC的下降相关;rs5219-rs2285676-rs5218位点组成的T-A-G单体型与HOMA-IR的下降相关,C-G-A单体型与TC指标的下降相关,rs5219-rs5218位点组成的C-A单体型与FPG的下降相关,rs2285676-rs5218位点组成的G-G单体型与HDL-C的升高相关。结论KCNJ11基因rs5219位点的CC基因型、rs2285676位点的GG基因型及部分单体型与运动干预前后糖脂代谢的不同指标变化有相关性,可作为评价糖尿病前期人群对运动干预敏感性的参考指标。
[Abstract]:Objective to study three tagged single nucleotide polymorphisms (SNPs) of potassium channel protein (KCNJ11) gene. The relationship between haplotype and related indexes of glucose and lipid metabolism before and after exercise intervention. Methods 70 patients with prediabetes mellitus were treated with aerobic endurance exercise for 12 weeks. The relative biochemical indexes of glucose and lipid metabolism were measured before and after intervention, and the genotype of genomic DNA polymorphic loci was analyzed by MALDI-TOF MS. Results the CC genotype of rs5219 locus and HOMA-IRT were correlated with the haplotype of single locus and multiple loci before and after intervention. The decrease of TG index was related to the increase of HDL-C index. The GG genotype of rs2285676 was correlated with the decrease of HbA1cHOMA-IRTC. The T-A-G haplotype of rs5219-rs2285676-rs5218 site was correlated with the decrease of HOMA-IR and C-G-A haplotype was correlated with the decrease of TC index. The C-A haplotype of rs5219-rs5218 site was correlated with the decrease of FPG. The G-G haplotype of rs2285676-rs5218 locus is associated with the increase of HDL-C. Conclusion the CC genotype of rs5219 locus of KCNJ11 gene is associated with the increase of HDL-C. The GG genotype and partial haplotype of rs2285676 locus were correlated with the changes of glucose and lipid metabolism before and after exercise intervention. It can be used as a reference index to evaluate the sensitivity of pre-diabetic population to exercise intervention.
【作者单位】：兰州城市学院体育学院;国营长风机器厂职工医院内科;兰州兰飞医院检验科;
【基金】：2014年度国家体育总局重点领域类项目(2014B025)
【分类号】：R587.1
【正文快照】： 糖尿病前期又称IGR,是机体糖代谢处于正常状态与糖尿病之间的阶段,包括IFG、IGT及二者的合并状态。糖尿病前期是T2DM发病过程中的必经阶段。它可持续存在,可转归为正常,也可转变为糖尿病。由于IGR有可逆性,因此,积极的干预措施是预防T2DM发生的关键。有氧运动是糖尿病前期人群

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