小麦分蘖角度TaTAC1基因同源克隆及表达分析

发布时间：2018-01-24 16:36

本文关键词： 小麦(Triticum aestivum L.) 分蘖角度 TaTAC 同源克隆表达分析　出处：《植物遗传资源学报》2017年01期 　论文类型：期刊论文

【摘要】：分蘖角度影响植物群体结构、光合效率以及植株的形态建成,最终影响其产量及品质。小麦中关于分蘖角度的研究鲜有报道。前人研究显示,水稻OsTAC1正向调控分蘖角度的大小,玉米Zm TAC1与叶片角度呈正相关。本研究的目的是解析Ta TAC1的表达模式并初步了解该基因的分子遗传机制及其与分蘖角度的遗传关系。本研究以CN16、SM969、Lan2399、SHW-1为材料,利用同源克隆分离获得Ta TAC1,利用生物信息学软件对Ta TAC1进行序列特征分析,应用实时荧光定量PCR对其表达模式进行分析,并进行Ta TAC1蛋白的亚细胞定位分析。结果显示,Ta TAC1长度约1.1~1.2 kb,包括780 bp的完整开放阅读框和320~370 bp的3'-UTR。Ta TAC1的c DNA序列分为2类,其中CN16-2、SM969-2的第10号碱基发生突变,引起终止子提前,导致Ta TAC1表达受阻;Lan2399-2和SHW-1-2在109~115碱基位置有"CGCGCG"片段插入,导致蛋白质β-折叠片减少。表达分析表明,Ta TAC1在分蘖期的叶鞘、茎高效表达,分蘖节其次,叶与根表达量最低。相关分析表明该基因在分蘖节各时期表达量与分蘖角度呈显著正相关,Pearson相关系数为0.677,其他组织表达量与分蘖角度无显著相关性。亚细胞定位分析显示Ta TAC1定位于细胞膜。从上述结果可以推测Ta TAC1在mRNA水平上正向调控分蘖角度,且可能参与生长素极性运输过程从而改变分蘖角度大小。
[Abstract]:Tiller angle affects plant population structure, photosynthetic efficiency and plant morphogenesis, and ultimately affects yield and quality. There are few reports on tiller angle in wheat. OsTAC1 positively regulated the tiller angle. Zm TAC1 was positively correlated with leaf angle. The expression pattern of TAC1 and the molecular genetic mechanism of the gene and its genetic relationship with tiller angle were preliminarily understood. CN16 was used in this study. Ta TAC-1 was isolated from SM969 / Lan2399 / SHW-1 by homologous cloning and sequenced by bioinformatics software. The expression pattern of Ta TAC1 protein was analyzed by real-time fluorescence quantitative PCR and the subcellular localization of Ta TAC1 protein was analyzed. The results showed that the length of Ta TAC1 was about 1.1 ~ 1.2kb. The complete open reading frame (ORAF) of 780bp and the c DNA sequence of 3GUTR.Ta TAC1 (320,370bp) were classified into two categories, including CN16-2. The mutation of the 10th base of SM969-2 caused the Terminator to advance and the expression of Ta TAC1 was blocked. The insertion of "CGCGCG" fragment of Lan2399-2 and SHW-1-2 at the 109N115base site resulted in the decrease of protein 尾 -folding tablets. Ta TAC1 was highly expressed in leaf sheath and stem at tillering stage, followed by tiller node and lowest in leaf and root. Correlation analysis showed that the expression of Ta TAC1 was positively correlated with tiller angle at each stage of tillering. Pearson correlation coefficient was 0. 677. There was no significant correlation between the expression of other tissues and tiller angle. Subcellular localization analysis showed that Ta TAC1 was located on cell membrane. From the above results, we can infer that Ta is located on cell membrane. The tiller angle was positively regulated by TAC1 at mRNA level. And it may be involved in the polar transport process of auxin, thus changing the tiller angle.
【作者单位】：四川农业大学小麦研究所分子生物学实验室;
【基金】：四川省科技支撑计划(2016NZ0057) 国家自然科学基金(31301317)
【分类号】：S512.1
【正文快照】： URL:http://www.cnki.net/kcms/detail/11.4996.S.20161214.1435.026.html小麦(Triticum aestivum L.)是世界一半以上人口的主要粮食,其种植面积、总产量以及总贸易额均居各类作物之首。合理的小麦株型可较大幅度提高产量,而分蘖角度是决定株型的主要因素之一,研究与之相关的

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