抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选

发布时间：2018-01-25 05:52

本文关键词： 水稻条纹叶枯病 STV 功能性标记粳型材料　出处：《中国水稻科学》2016年06期 　论文类型：期刊论文

【摘要】：条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11 KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料[5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份{9锦/Habataki//{9锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S(感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。
[Abstract]:Striped leaf blight is one of the most serious disease in Oryza sativa L.). STV11 is a resistance gene of stripe blight identified from Kasalath of indica rice. According to the functional polymorphism of STV11 KAS6 deletion of Kasalath type stripe disease gene. A functional molecular marker qstv11 based on PCR was designed. [Five resistant cultivars and five susceptible varieties were collected from 17 japonica japonica lines of 17 Japanese japonica lines (population A), and 5 susceptible varieties were collected from Liaoning Province, and were collected from 42 Japanese japonica inbred lines (population A), which were collected from Qiuguang / Qishanzhan inbred lines. 85 {9 brocade / Habataki / {9 brocade backcross recombinant inbred lines (population BX, population parent 4) were analyzed for genotype and identification of disease resistance. Qstv11 was able to distinguish susceptible genotypes, and one of 41 Liaoning materials was identified as STV11-R (resistance). The loci of Japonica japonica were STV11-S( allelic). In population A and B, 24 and 6 japonica rice background STV11-R materials were screened, respectively.
【作者单位】：辽宁省盐碱地利用研究所;
【基金】：辽宁省农业领域青年科技创新人才培养计划资助项目(2015035,2015036)
【分类号】：S435.111.4
【正文快照】： Development of Functional Marker for Rice stripe virus Resistant Gene STV11and Resistant Germplasm Selection in japonica RiceMAOTing,LI Xu,ZHANGZhan,FULi-dong,LI Zhen-yu*(Liaoning Province Saline and Alkaline Land Utilization and Research Institute,Panji

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