利用Pigm基因改良粳稻保持系的稻瘟病抗性研究

发布时间：2018-01-25 11:03

本文关键词： 水稻稻瘟病分子标记辅助选择 Pigm基因　出处：《核农学报》2017年03期 　论文类型：期刊论文

【摘要】：选育和应用抗病品种是防治水稻稻瘟病经济有效的技术途径。为了改良粳稻保持系浙04B的稻瘟病抗性,以含稻瘟病广谱抗性基因Pigm的粳稻材料MP3为供体亲本,浙04B为受体亲本,通过杂交、回交并结合分子标记辅助选择,将Pigm基因导入浙04B中,利用分布于水稻12条染色体上的47个多态性SSR标记检测近等基因系的遗传背景回复率,获得了23份背景回复率0.80的近等基因系。选用4个浙江省稻瘟病优势菌株Zj-5、Zj-8、13-37和13-41人工接种鉴定,23份近等基因系均表现为抗穗颈瘟。田间自然诱发鉴定结果表明,23份近等基因系叶瘟为0级,穗瘟均为1级,表现为抗穗瘟,表明近等基因系稻瘟病抗性明显提高。对10项农艺性状的考察结果表明,导入Pigm基因的近等基因系剑叶变短,千粒重增加,生育期延迟,株高增加,有效穗数、每穗总粒数和产量总体减少。本研究结果为选育抗稻瘟病新品种提供了材料,同时为Pigm基因抗病育种应用提供了参考。
[Abstract]:Breeding and application of disease-resistant varieties is an economical and effective technical way to control rice blast in order to improve rice blast resistance of japonica rice maintainer line Zhejiang 04B. Japonica rice (MP3) containing the broad spectrum resistance gene (Pigm) of rice blast was used as donor parent and Zhejiang 04B as recipient parent. The Pigm gene was introduced into Zhejiang 04B and 47 polymorphic SSR markers distributed on 12 rice chromosomes were used to detect the recovery rate of genetic background of near-isogenic lines. 23 near-isogenic lines with a response rate of 0.80 were obtained. Four dominant strains of rice blast in Zhejiang Province, Zj-5, Zj-8J-83, 13-37 and 13-41, were identified by artificial inoculation. All the 23 near-isogenic lines showed resistance to panicle blast. The results of field induced identification showed that leaf blast of 23 near-isogenic lines was grade 0 and panicle blast was grade 1, which showed resistance to panicle blast. The results showed that the resistance of near-isogenic lines to rice blast was significantly increased. The results of 10 agronomic traits showed that the leaf of the near-isogenic lines with Pigm gene was shorter, the 1000-grain weight was increased, the growth period was delayed, and the plant height was increased. The number of effective panicles, the total grains per panicle and the yield were decreased. The results provided a reference for breeding new varieties resistant to rice blast and for the application of Pigm gene in disease resistance breeding.
【作者单位】：浙江省农业科学院作物与核技术利用研究所;
【基金】：转基因专项(2015ZX08009-003-001) 浙江省农科院科研类专项经费(2012R16Y01E01) 浙江省重大专项(8812计划)
【分类号】：S435.111.41
【正文快照】： 水稻(Oryza sativa L.)稻瘟病(rice blast)是由稻瘟病菌(Magnaporthe oryzae)引起的最具毁灭性的水稻真菌性病害之一,可导致水稻减产,严重时减产幅度达40%~50%,甚至颗粒无收[1-2]。因此,稻瘟病是制约水稻生产的重要因素,在现行的水稻品种审定条例中,稻瘟病抗性是一项重要的评

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