山羊Wnt6基因克隆及组织表达分析

发布时间：2018-01-25 21:36

本文关键词： 山羊 Wnt基因克隆组织表达荧光定量PCR　出处：《黑龙江畜牧兽医》2017年13期 　论文类型：期刊论文

【摘要】：为了研究Wnt6基因在简州大耳羊不同器官组织中的表达水平,试验采用RT-PCR方法克隆山羊Wnt6基因序列,采用荧光定量PCR技术检测该基因在各器官组织中的表达情况。结果表明:获得山羊Wnt6基因序列1 120 bp(Gen Bank登录号为KU950833),其中CDS为1 098 bp,5'UTR16 bp和3'UTR 6 bp,编码365个氨基酸,存在1个信号肽剪切位点,跨膜序列为7~29位氨基酸。氨基酸同源性比较发现,山羊Wnt6与绵羊和牛的同源性最高达99%;山羊Wnt6基因在心脏、肝脏、脾脏、肺脏、肾脏、脂肪、背最长肌、股二头肌、臂三头肌中存在广泛表达,且在脂肪组织中的表达水平极显著高于其他器官组织(P0.01)。
[Abstract]:In order to study the expression level of Wnt6 gene in different organs and tissues of large ear sheep in Jianzhou, RT-PCR method was used to clone goat Wnt6 gene sequence. Fluorescence quantitative PCR technique was used to detect the expression of the gene in various organs and tissues. The results showed that the goat Wnt6 gene sequence was 1 120 BP (. Gen Bank login number is KU950833). The CDS was 1 098 BP / 5 UTR16 BP and 3 UTR6 BP, encoding 365 amino acids, and there was a signal peptide splicing site. The transmembrane sequence was 7 ~ 29 amino acids. The homology of amino acid homology between goat Wnt6 and sheep and cattle was 999.The results showed that goat Wnt6 had the highest homology with sheep and cattle. Goat Wnt6 gene is widely expressed in heart, liver, spleen, lung, kidney, fat, longissimus dorsi muscle, biceps femoris muscle and triceps arm muscle. The expression level in adipose tissue was significantly higher than that in other organs.
【作者单位】：西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：四川省“十三五”畜禽育种攻关项目(2016NZ0099-36) 四川省科技创新产业链示范工程重大项目(2014NZ0003) 西南民族大学研究生“创新型科研项目”(CX2016SZ081)
【分类号】：S827
【正文快照】： 资源保护与利用四川省重点实验室,成都610041)Wnt(Wingless-Type MMTV Integration Site Fam-ily)蛋白是一种由Wnt基因编码的能调控不同发育过程的分泌型蛋白家族,参与了细胞增殖、细胞极性、细胞迁移和细胞分化等多种发育过程[1]。T.C.Pres-twich等[2]研究表明,Wnt存在经典和

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