肺炎链球菌分离株的耐药性与毒力基因研究

发布时间：2018-01-26 00:47

本文关键词： 肺炎链球菌耐药性毒力基因　出处：《中华医院感染学杂志》2017年23期 　论文类型：期刊论文

【摘要】：目的探讨新生儿肺炎链球菌分离株耐药性、毒力基因的携带状况与菌株间的亲缘性。方法选择2012年1月-2016年12月期间于医院分娩的未曾出院的新生儿肺炎患儿分离出的39株肺炎链球菌,采用pbp2B种特异聚合酶链反应(PCR)检测判定肺炎链球菌,采用纸片扩散法(K-B法)测定药物敏感性,采用PCR及序列分析的方法分析5种耐药基因(pbp2B突变型、ermB、mefA、tetM、intTn)与10种毒力基因(pspA、pspC、rrgA、nanA、pavA、iga、hysA、lytA、lytB、ply),并对检测结果作样本聚类分析。结果 39株肺炎链球菌对青霉素、红霉素、阿奇霉素、四环素的耐药率均89.0%,对头孢噻肟、万古霉素、替考拉宁均敏感;39株菌5种耐药基因均有检出,且检出率非常高,大环内酯类药物耐药基因总检出率达100.0%,并检出7种毒力基因;样本聚类分析提示39株菌存在9个小克隆,但具有多样性,9个小克隆分别为1-34-39号株、6-35号株、3-10-19号株、13-16-14号株、15-17-18号株、23-30号株、36-37号株、24-25-26号株、4-28号株。结论本组菌株中检出pbp2B突变型、ermB、mefA、tetM、intTn等5种耐药基因是导致本组菌对青霉素、大环内酯类药物和四环素耐药率高的重要原因,耐药表型与基因型基本对应;分离株携带的6种毒力基因有黏附、侵袭、自溶、细胞毒、生物膜形成等毒力作用。
[Abstract]:Objective to investigate the drug resistance of Streptococcus pneumoniae isolates from neonates. Methods 39 strains of Streptococcus pneumoniae were isolated from newborns who had not been discharged from hospital from January 2012 to December 2016. . Streptococcus pneumoniae was detected by pbp2B species-specific polymerase chain reaction (PCR), and the drug sensitivity was determined by disk diffusion method (K-B method). PCR and sequence analysis were used to analyze 5 resistant genes (Pbp2B) and 10 virulence genes (pspA). PspCr rg An Aconia pavilion igaa hyshyt Alyt Bu plyy. Results 39 strains of Streptococcus pneumoniae were resistant to penicillin, erythromycin, azithromycin and tetracycline, and were resistant to cefotaxime and vancomycin. Teicoplanin was sensitive; The detection rate of 5 drug resistance genes in 39 strains was very high. The total detection rate of macrolide resistance genes was 100.0 and 7 virulence genes were detected. Sample cluster analysis showed that 39 strains had 9 small clones, but had diversity. The nine clones were 1-34-39 strain No. 6-35 and strain No. 3-10-19, respectively. Strain 13-16-14, strain No. 15-17-18, strain No. 23-30, strain No. 36-37, strain No. 24-25-26. Conclusion five resistant genes of pbp2B mutagenesis were found to be the cause of penicillin in this group. The main reason for the high resistance rate of macrolides and tetracycline was that the phenotype of resistance to macrolides and tetracycline basically corresponded to genotype. The 6 virulence genes carried by the isolates had the virulence of adhesion, invasion, autolysis, cytotoxicity and biofilm formation.
【作者单位】：宁波大学医学院附属医院呼吸科;宁波大学医学院附属医院儿内科;宁波大学医学院附属医院ICU病房;宁波大学医学院附属医院细菌室;
【基金】：宁波市自然科学基金资助项目(2016A610132) 宁波市医学科技基金资助项目(2011B13)
【分类号】：R446.5;R722.135
【正文快照】： 肺炎链球菌最早由巴斯德(Louis Pasteur)及Sternberg分别在法国及美国从肺炎患者的痰液中首次分离出(1881年)。为革兰染色阳性,菌体似矛头状,成双或成短链状排列的双球菌,曾被命名为肺炎双球菌。肺炎链球菌是健康人上呼吸道的定植菌,但同时也是具毁灭性的病原菌(即可致患者死

【相似文献】