蓼科大黄属植物CHS基因密码子偏爱性分析

发布时间：2018-01-26 07:14

本文关键词： 大黄属CHS基因密码子偏爱性 GC GC ENC 最优密码子　出处：《分子植物育种》2017年06期 　论文类型：期刊论文

【摘要】：为探究蓼科大黄属物种CHS基因密码子的偏爱性,鉴定出该类基因的最优密码子。利用EMBOSS、Codon W1.4.4和SPSS 17.0软件对24条来源于大黄属不同物种的CHS基因进行多元统计分析,以深入研究其偏爱性。结果表明,大黄属CHS基因密码子的GC_3含量最高,其与GC_(12)的相关性检验未达到显著水平。ENC-plot分析显示,24条CHS基因的ENC值均低于预期值。以上证明了自然选择是CHS基因密码子偏爱性形成的主导因素。筛选出的26个最优密码子中,17个密码子的第3位碱基为G/C,表明CHS基因最优密码子偏爱以G/C结尾。
[Abstract]:In order to investigate the codon preference of CHS gene in Rheum species of Polygonaceae, the optimal codon of this kind of gene was identified. EMBOSS was used. Codon W1.4.4 and SPSS 17.0 software were used to analyze 24 CHS genes from different species of Rhubarb in order to study their preference. The GC_3 content of CHS gene codon was the highest in Rhubarb, and the correlation test between GC_3 and GCS-12) did not reach the significant level. ENC-plot analysis showed that the codon content of rhubarb gene was the highest. The ENC values of 24 CHS genes were all lower than the expected values, which proved that natural selection was the leading factor in the formation of codon preference of CHS gene. The third base of 17 codon is G / C, which indicates that the optimal codon of CHS gene ends with G / C.
【作者单位】：四川农业大学生命科学学院;
【基金】：四川农业大学生命科学学院学科建设双支项目,四川农业大学双支计划共同资助
【分类号】：Q943.2
【正文快照】： The Codon Usage Analysis in CHS Gene within the Polygonaceae RheumMa Shengchao Yu Xinmei Yao Huipeng*College of Life Science,Sichuan Agricultural University,Ya'an,625014*Corresponding author,yaohuipeng0921@163.comYuzhong(Molecular Plant Breeding),15(6):2

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