亚抑菌浓度头孢他啶对大肠埃希菌胞外多糖和pga基因的影响

发布时间：2018-01-26 15:47

本文关键词： 大肠埃希菌头孢他啶生物膜亚抑菌浓度胞外多糖 pga基因　出处：《解放军药学学报》2016年06期 　论文类型：期刊论文

【摘要】：目的明确亚抑菌浓度头孢他啶在大肠埃希菌生物膜抑制过程中对胞外多糖和pga基因的影响。方法采用标准琼脂平板倍比稀释法检测细菌最低抑菌浓度,采用96孔板结晶紫染色法检测生物膜形成能力,采用苯酚-硫酸法测定胞外多糖含量,pgaABCD基因表达采用RT-PCR扩增。结果大肠埃希菌对氨苄西林和氟喹诺酮类耐药率较高,而对阿米卡星和碳青霉烯类全部敏感。亚抑菌浓度头孢他啶对大肠埃希菌生物膜形成、胞外多糖产生能力和pgaABCD基因表达均有显著抑制作用,但其降低程度不完全一致。结论亚抑菌浓度头孢他啶在抑制生物膜形成过程中能降低大肠埃希菌胞外多糖产生和pga基因表达,但其与生物膜降低程度存在差异性。
[Abstract]:Objective to investigate the influence of subinhibitory concentrations of ceftazidime in Escherichia coli biofilm inhibition of extracellular polysaccharide and PGA gene. Methods using standard agar plate detection times higher than the minimum inhibitory concentration dilution method, forming ability by 96 hole plate biofilm by crystal violet staining, cell was determined by phenol sulfuric acid method outside the polysaccharide content, the expression of pgaABCD gene was amplified by RT-PCR. The higher rate of Escherichia coli to ampicillin and fluoroquinolones, and all sensitive to Amikacin and carbapenems. Subinhibitory concentrations of ceftazidime on Escherichia coli biofilm formation, exopolysaccharide production ability and pgaABCD gene expression were significantly inhibited the degree of decrease, but not completely consistent. Conclusion subinhibitory concentrations of ceftazidime in inhibiting biofilm formation in Escherichia coli can reduce the exopolysaccharide production and PGA gene expression But it is different from the reduction of biofilm.

【作者单位】：第三军医大学西南医院药剂科;第三军医大学新桥医院药剂科;
【基金】：国家自然科学基金项目,No.81373451
【分类号】：R96
【正文快照】： 生物膜是细菌黏附于生物或非生物表面形成的具有立体结构的细胞群落[1]。大肠埃希菌是生物膜感染的常见病原菌,形成生物膜的大肠埃希菌耐药急剧增加,一直是临床治疗的难题[2]。随着医疗科学和技术的发展,更多危重患者得到有效治疗,同时静脉导管、气管插管和尿管的使用频率和时

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