寄主诱导的基因沉默提高植物真菌病害抗性研究进展

发布时间：2018-01-26 20:26

本文关键词： RNA干扰寄主诱导的基因沉默(HIGS) 真菌病害转基因植物　出处：《作物学报》2017年08期 　论文类型：期刊论文

【摘要】：寄主诱导的基因沉默(host-induced gene silencing,HIGS)以病原菌生长发育、产孢繁殖、侵染或致病过程中的关键基因作为靶点,在寄主植物中表达针对靶基因的RNAi构建体,在病原菌侵染植物的过程中,摄入相应的ds RNA或si RNA分子,通过识别、结合特异核苷酸序列,干扰病菌靶基因表达,从而抑制病菌侵染和扩展,使植物表现抗病。这项技术为培育基于病原菌特异序列的植物抗病性奠定了基础,显示了巨大的应用潜力。本文综述了利用HIGS技术提高植物真菌病抗性的最新研究进展,总结了国内外利用这项技术改良植物真菌病害抗性的主要策略、技术路线,展望了发展应用前景。
[Abstract]:Host-induced gene silencing (HIGSs) was induced by host gene. The key genes in the process of infection or pathogenicity are used as targets to express the target gene RNAi construction in host plants, and in the process of pathogen infecting plants. Intakes the corresponding DS RNA or si RNA molecule, through the recognition, binds the specific nucleotide sequence, interferes the pathogen target gene expression, thus inhibits the pathogen infection and the expansion. This technique lays the foundation for the cultivation of plant disease resistance based on the specific sequence of pathogens. This paper reviews the latest research progress in improving plant fungal disease resistance by using HIGS technology, and summarizes the main strategies to improve plant fungal disease resistance using this technique at home and abroad. The technical route and prospect of development and application are prospected.
【作者单位】：福建农林大学作物科学学院/作物遗传改良和综合利用教育部重点实验室;华中农业大学植物科学技术学院/麦类作物分子生物技术实验室;
【基金】：国家转基因生物新品种培育重大专项(2016ZX08002001-003) 国家自然科学基金项目(31601761) 福建省中青年教师教育科研项目(JAT160180)资助~~
【分类号】：S432.44
【正文快照】： 本研究由国家转基因生物新品种培育重大专项(2016ZX08002001-003),国家自然科学基金项目(31601761)和福建省中青年教师教育科研项目(JAT160180)资助。This research was supported by the National Major Project for Developing New GM Crops(2016ZX08002001-003),the Nation

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