Rb94基因联合放射治疗对荷瘤裸小鼠食管癌细胞生长的抑制作用

发布时间：2018-01-28 22:46

本文关键词： Rb基因放射治疗荷瘤裸小鼠食管肿瘤　出处：《吉林大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨人视网膜母细胞瘤Rb94基因联合电离辐射对荷瘤裸小鼠食管癌细胞生长的抑制作用,阐明Rb94基因与放射治疗(放疗)抑制肿瘤细胞生长的协同作用。方法:将食管癌K150细胞接种于BALB/c-nu裸小鼠建立肿瘤模型。实验分为对照组(未进行处理)、Ad-LacZ组(为含LacZ基因但不含Rb94基因的对照腺病毒,分别于0、3和7d以Ad-LacZ感染瘤体)、Ad-Rb94组(分别于0、3和7d以Ad-Rb94感染瘤体)、照射组(分别于1、4和8d进行4Gyγ射线局部照射瘤体)和Ad-Rb94联合照射组(联合组,Ad-Rb94感染后进行4Gyγ射线局部照射瘤体)。检测各组小鼠食管癌瘤体体积、瘤体质量、肿瘤生长抑制率和肿瘤组织中ABL及JNK的表达水平,并对肿瘤组织进行病理学分析。结果:与对照组比较,Ad-Rb94组、照射组和联合组裸小鼠肿瘤生长速度缓慢,肿瘤生长出现抑制效应;治疗第15天联合组小鼠肿瘤体积明显小于Ad-Rb94组和照射组,与对照组和Ad-LacZ组比较差异有统计学意义(F=26.7,F=23.8,P0.01)。治疗结束后联合组小鼠瘤体质量最轻,肿瘤生长抑制率高达81.16%,明显高于Ad-Rb94组(57.84%)和照射组(38.20%)(P0.01)。与对照组比较,联合组小鼠肿瘤组织中ABL和JNK的表达水平明显升高(P0.01)。与其他组比较,联合组肿瘤细胞有较少的核分裂和较浅的核深染。结论:Rb94基因联合放疗对荷瘤裸小鼠肿瘤的生长抑制具有协同作用。
[Abstract]:Objective: to investigate the inhibitory effect of Rb94 gene combined with ionizing radiation on the growth of esophageal carcinoma cells in nude mice bearing human retinoblastoma. Elucidation of Rb94 gene and radiotherapy (radiotherapy). Methods: K150 cells of esophageal carcinoma were inoculated into BALB/c-nu nude mice to establish tumor model. The experiment was divided into control group (untreated). Ad-LacZ group (control adenovirus containing LacZ gene but not Rb94 gene) was infected with Ad-LacZ on the 3rd and 7th day respectively. The tumor was infected with Ad-Rb94 for 3 and 7 days. The irradiated group (4 Gy 纬 -ray local irradiation on the 4th and 8th day respectively) and the Ad-Rb94 combined group (combined group). After Ad-Rb94 infection, local irradiation of 4Gy 纬 -ray was carried out. The tumor volume and tumor mass were measured in each group of mice with esophageal carcinoma. The tumor growth inhibition rate and the expression of ABL and JNK in tumor tissue were analyzed by pathology. Results: compared with the control group, the expression of ABL and JNK in Ad-Rb94 group was compared with that in control group. The tumor growth rate of nude mice in irradiation group and combined group was slow and the tumor growth was inhibited. On the 15th day of treatment, the tumor volume in the combined group was significantly smaller than that in the Ad-Rb94 group and the irradiation group, and the difference was statistically significant compared with the control group and the Ad-LacZ group. After the treatment, the tumor mass of the combined group was the lightest, and the tumor growth inhibition rate was as high as 81.16%. It was significantly higher than that in the Ad-Rb94 group (57.84) and the irradiation group (38.20%) compared with the control group. The expression of ABL and JNK in the tumor tissues of the combined group was significantly higher than that of the other groups. Conclusion the combination of Rb94 gene and radiation therapy has synergistic effect on tumor growth inhibition of nude mice bearing tumor. [WT5HZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [
【作者单位】：中国医学科学院放射医学研究所天津市放射医学与分子核医学重点实验室;
【基金】：国家自然科学基金资助课题(30770638) 北京协和医学院教学改革项目资助课题(院1444)
【分类号】：R730.5
【正文快照】： 视网膜母细胞瘤(retinoblastoma,Rb)是婴幼儿内眼恶性肿瘤中最常见的一种,其发病率在婴幼儿中为1∶28 000~1∶15 000[1-3]。视网膜母细胞瘤的形成是由于第13号染色体上(13q14)一对等位基因的同时缺失或失活所致,Rb基因为肿瘤抑制基因或抗癌基因,具有抑制肿瘤形成的作用。N末端

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