咖啡碱和茶多酚组合对小鼠脂肪酸合成酶基因和蛋白表达的影响

发布时间：2018-01-29 04:59

  本文关键词： 咖啡碱和茶多酚组合 脂肪沉积 脂肪酸合成酶 减肥　出处：《中国食品学报》2016年09期 　论文类型：期刊论文

【摘要】：探讨咖啡碱和茶多酚组合对小鼠肝脏脂肪酸合成酶(FAS)基因和蛋白表达的影响,阐明其减肥机制。50只昆明小鼠随机被分成5组,分别为对照组(I)、0.03%咖啡碱+0.3%茶多酚(II)、0.03%咖啡碱+0.6%茶多酚(III)、0.06%咖啡碱+0.3%茶多酚(IV)、0.06%咖啡碱+0.6%茶多酚(V),喂养12周。分析肝脏FAS的m RNA和蛋白表达量。咖啡碱与茶多酚组合的小鼠体重增加与对照组相比均有下降趋势,特别是V组的小鼠体重增加显著减少,咖啡碱与茶多酚组合的小鼠腹腔内脂肪(IPAT)质量均明显减少。IV、V组的血中TG、TC浓度显著降低,II、III组的NEFA浓度明显降低。与对照组相比,咖啡碱和茶多酚组合FAS m RNA表达量均显著降低,IV和V组的FAS蛋白表达量显著减少。以上结果表明咖啡碱与茶多酚组合通过调控小鼠FAS的m RNA及蛋白表达量,抑制脂肪合成,降低血脂浓度,引起体内脂肪沉积减少,达到预防肥胖的效果。
[Abstract]:To investigate the effect of caffeine and tea polyphenols on the expression of FASgene and protein in mouse liver fatty acid synthase, 50 Kunming mice were randomly divided into 5 groups. The control group was 0.03% caffeine 0.3% tea polyphenols, 0.03% caffeine 0.6% tea polyphenols. 0.06% caffeine 0.3% tea polyphenols 0.06% caffeine 0.6% tea polyphenols V). After 12 weeks of feeding, the expression of m RNA and protein in liver FAS was analyzed. The weight gain of mice combined with caffeine and tea polyphenols showed a decreasing trend compared with the control group. Especially the weight gain of mice in group V was significantly decreased, and the weight of IP ATA of mice combined with caffeine and tea polyphenols was significantly decreased. The concentration of TGN TC in blood of group V was significantly lower than that of group V. Compared with the control group, the expression of FAS m RNA in caffeine and tea polyphenols was significantly decreased. The results showed that the combination of caffeine and tea polyphenols could inhibit fat synthesis and decrease the concentration of lipid by regulating the expression of m RNA and protein of FAS in mice. Causes the body fat deposition to reduce, achieves the prevention obesity effect.
【作者单位】： 江西农业大学江西省天然产物与功能食品重点实验室;江西农业大学图书馆;
【基金】：国家自然科学基金项目(No.31160320) 教育部留学回国人员科研启动金(教外司留[2009]1001号) 江西省自然科学基金项目(No.20142BAB204003)
【分类号】：R589.2
【正文快照】： 绿茶是亚洲消费量较多的饮料之一,有许多生理功效,其主要功能成分是咖啡碱和茶多酚。咖啡碱有抗炎症作用[1],骨髓基质细胞株分化诱导成成骨细胞,并促进骨细胞中无机盐沉积作用,增加骨密度[2],预防非酒精型脂肪肝[3]。咖啡碱被证明能提高肥胖人群的能量代谢、脂肪燃烧及静息代，

本文编号：1472593