玉米WRKY基因功能分析

发布时间：2018-01-30 03:25

本文关键词： 玉米 WRKY转录因子功能分析非生物胁迫　出处：《南方农业学报》2017年06期 　论文类型：期刊论文

【摘要】：【目的】分析WRKY基因在玉米生长发育及逆境胁迫下的功能,为阐明WRKY家族基因在玉米生长和逆境响应机制中的功能和作用打下基础。【方法】利用生物信息学技术从玉米基因组中得到3个进化关系较近的WRKY基因,应用在线预测软件对3个基因的功能和结构进行分析,并运用荧光定量RT-PCR(qRT-PCR)分析3个基因在玉米不同组织及在高盐、低温和干旱胁迫下的表达模式。【结果】从玉米基因组中得到ZmWRKY22-like、ZmWRKY55-like和ZmWRKY74-like 3个玉米WRKY转录因子家族基因,预测表明3个蛋白均定位于细胞核。荧光定量PCR分析结果表明,3个基因在玉米的不同器官中均有表达,但具有组织表达特异性。高盐处理24 h,ZmWRKY55-like基因的表达量上升为对照的4.5倍;低温处理24 h,ZmWRKY74-like基因的表达量上升为对照的2.1倍。【结论】ZmWRKY22-like、ZmWRKY55-like和ZmWRKY74-like基因可能在玉米果实发育过程中起到一定作用,其中ZmWRKY55-like和ZmWRKY74-like基因可能分别参与植物对盐和低温胁迫的响应。
[Abstract]:[objective] to analyze the function of WRKY gene in maize growth and development and stress stress. In order to elucidate the function and function of WRKY family genes in maize growth and stress response mechanism, three WRKY with close evolutionary relationship were obtained from maize genome by bioinformatics technique. Genes. The function and structure of the three genes were analyzed by on-line prediction software, and the three genes were analyzed in different tissues and high salt by RT-PCRX qRT-PCRR. Expression patterns under low temperature and drought stress. [results] ZmWRKY22-like was obtained from maize genome. WRKY transcription factor family genes of ZmWRKY55-like and ZmWRKY74-like in maize. The results of fluorescence quantitative PCR analysis showed that the three genes were expressed in different organs of maize, but had tissue expression specificity, and were treated with high salt for 24 h. The expression of ZmWRKY55-like gene was 4.5-fold higher than that of the control. The expression of ZmWRKY74-like gene increased 2.1-fold as compared with that of the control at 24 h after hypothermia treatment. [conclusion] ZmWRKY22-like. ZmWRKY55-like and ZmWRKY74-like genes may play a role in the development of maize fruit. ZmWRKY55-like and ZmWRKY74-like genes may be involved in the response of plants to salt and low temperature stress, respectively.
【作者单位】：中国热带农业科学院南亚热带作物研究所;农业部热带果树生物学重点实验室;
【基金】：国家自然科学基金项目(31572087) 海南省自然科学基金项目(20163111)
【分类号】：Q943.2;S513
【正文快照】： 0引言【研究意义】WRKY转录因子是近年来在植物中发现的一类重要转录因子,因其N-末端含有高度保守的WRKYGQK氨基酸序列而得名,是高等植物中10个最大转录因子家族之一。WRKY蛋白的N-端均含有1个或2个由近60个氨基酸组成的WRKY结构域,C-(1South Subtropical Crops Research Inst

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