棘球蚴基因工程亚单位疫苗免疫绵羊的效果研究

发布时间：2018-01-30 12:46

本文关键词： 棘球蚴病棘球蚴基因工程亚单位疫苗免疫研究　出处：《中国兽医杂志》2017年08期 　论文类型：期刊论文

【摘要】：采用羊棘球蚴基因工程亚单位疫苗对2 315只3~20月龄藏系绵羊进行3次免疫试验,并用间接ELISA对免疫后的抗体进行动态监测。检测结果显示,首免前、首免1周后、二免1周后、二免1年后、三免1周后、三免后6个月的血清抗体阳性率依次为0%、98.75%、94.94%、40.74%、98.72%、50%。同时,对同龄、同群、同草场放牧的羊三免1年后进行剖检,免疫组59只、对照组60只。结果显示,对照组感染率、感染强度为86.67%和3.43,免疫组感染率、感染强度为45.76%和0.86,免疫保护率(减囊率)为69.38%。
[Abstract]:A total of 2 315 Tibetan sheep aged 3 to 20 months were immunized with Echinococcus Sheep gene engineering subunit vaccine for 3 times. Indirect ELISA was used to monitor the antibody after immunization. The results showed that the first immunity was before, the first one week, the second one week, the second one year, and the third one week. The positive rate of serum antibody at 6 months after three immunizations was 0 and 98.75 and 94.940.74 respectively. At the same time, for the same age, the same group. A year later, 59 sheep were killed in the immunized group and 60 in the control group. The results showed that the infection rate in the control group was 86.67% and 3.43, and the infection rate in the immunized group was 86.67% and 3.43 respectively. The infection intensity was 45.76% and 0.86, and the immune protective rate was 69.38.
【作者单位】：青海省动物疫病预防控制中心;青海省贵南县动物疫病预防控制中心;青海省泽库县动物疫病预防控制中心;
【基金】：中国动物疫病预防控制中心“棘球蚴病综合防控技术集成与示范项目”
【分类号】：S858.26
【正文快照】： 棘球蚴病(Echinoccosis)俗称包虫病(Hydatido-sis),是一种人兽共患寄生虫病。据调查,在我国西北畜牧业发达地区的人群血清阳性率为1.06%~32%,患病率为1.0%~7.64%[1],而我国动物感染棘球蚴病每年造成的畜产品经济损失高达8亿元以上[2]。青海省是我国棘球蚴病高发流行区,家畜和

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