全天麻及天麻素联合卡马西平对癫痫小鼠脑部海马体神经元及基因mrp1表达的影响

发布时间：2018-02-01 15:42

本文关键词： 全天麻胶囊天麻素卡马西平多药耐药相关蛋白基因癫痫　出处：《中国新药杂志》2017年13期 　论文类型：期刊论文

【摘要】：目的:本研究旨在说明全天麻胶囊及天麻素联合卡马西平应用于抗癫痫治疗后,对于卡马西平抗癫痫作用的影响及作用机制。方法:通过戊四唑诱导的癫痫小鼠模型,采用癫痫模型发作等级评分,对不同给药组癫痫模型小鼠进行评分,以判断联合给药后卡马西平抗癫痫作用。通过普通病理学、RT-PCR研究全天麻胶囊及天麻素联合卡马西平对癫痫小鼠脑部海马体神经元及多药耐药相关蛋白基因(mrp1)的影响。结果:全天麻及天麻素联合卡马西平能够显著提高卡马西平的抗癫痫作用,全天麻胶囊可改善海马CA3区神经元损伤,而天麻素及卡马西平则无该类作用。全天麻胶囊可逆转因多次给药引起的mrp1基因的高表达,天麻素则会在多次给药后诱导mrp1基因高表达。结论:全天麻胶囊可提高卡马西平的抗癫痫小鼠作用,与减轻海马神经元损伤及降低mrp1基因高表达相关。
[Abstract]:Objective: the purpose of this study was to demonstrate that Gastrodia elata capsule and Gastrodin combined with carbamazepine were used after antiepileptic therapy. Methods: the epileptic mice model induced by pentylenetetrazole was used to evaluate the epileptic model mice in different administration groups. To judge the antiepileptic effect of carbamazepine after combined administration. RT-PCR study on the effects of Gastrodin and Gastrodin combined with carbamazepine on hippocampal neurons and multidrug resistance-associated protein gene mrp1 in epileptic mice. Results: whole day and Gastrodin combined with carbamazepine could significantly improve the antiepileptic effect of carbamazepine. Gastrodin and carbamazepine can reverse the overexpression of mrp1 gene induced by multiple administration of Gastrodia elata capsule, but Gastrodin and carbamazepine have no such effect. Gastrodin can induce high expression of mrp1 gene after repeated administration. Conclusion: Quantianma capsule can improve the antiepileptic effect of carbamazepine in mice. It is related to reducing the injury of hippocampal neurons and reducing the overexpression of mrp1 gene.
【作者单位】：兰州大学第二医院药学中心;中山大学药学院;
【基金】：中央高校科研基金资助项目(862434)
【分类号】：R965
【正文快照】： 癫痫是一种由大脑神经元异常性放电引起的会导致大脑短暂功能障碍的疾病[1]。目前抗癫痫药物有几十种,约2/3的患者均能通过这类药物的治疗,达到控制的目的,但仍有1/3的患者无法得到有效的治疗,进一步发展成为难治性癫痫或者顽固性癫痫[2]。临床治疗中,若一种抗癫痫药物不能有

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