雷竹VRN1同源基因克隆及功能分析

发布时间：2018-02-02 14:27

本文关键词： 雷竹 PvVRN 亚细胞定位功能分析　出处：《核农学报》2016年09期 　论文类型：期刊论文

【摘要】：拟南芥VERNALIZATION 1(VRN1)基因是一个介导春化途径,调控开花时间的基因。为了研究竹类植物中VRN1同源基因的功能,采用同源克隆技术从雷竹中分离到一个VRN1同源基因,将其命名为PvVRN1。序列分析表明,该基因编码245个氨基酸,与小麦、水稻中VRN1同源基因相似性分别为86.56%和88.98%。实时荧光定量PCR结果表明,PvVRN1在开花雷竹中的表达量高于未开花雷竹;在开花雷竹中,PvVRN1在秆、花、叶芽中表达量相对较高。亚细胞定位分析表明,PvVRN1蛋白质主要存在细胞核中。PvVRN1在拟南芥中的过表达使转基因植株矮小,花瓣和萼片比野生型短,但是在开花时间上与野生型无差异,表明PvVRN1基因可能参与雷竹花形态建成以及其它发育过程。本研究结果可为阐明竹子开花机制提供科学依据。
[Abstract]:Arabidopsis thaliana VERNALIZATION 1 VERNALIZATION 1) gene is a gene that mediates vernalization and regulates flowering time. In order to study the function of VRN1 homologous gene in bamboo plants. A VRN1 homologous gene was isolated from Phyllostachys aestivum by homologous cloning technique and named PvVRN1.The sequence analysis shows that the gene encodes 245 amino acids, which is similar to that of wheat. The similarity of VRN1 homologous genes in rice was 86.56% and 88.98, respectively. The results of real-time fluorescence quantitative PCR showed that the expression of PvVRN1 in flowering phyllostachys phyllostachys was higher than that in unblooming Phyllostachys phyllostachys. The expression of PvVRN1 was relatively high in stem, flower and leaf bud. The subcellular localization analysis showed that PvVRN1 was expressed in the flower of Phyllostachys praecox. The over-expression of PvVRN1 protein in the nucleus of Arabidopsis caused the transgenic plants to be short, the petals and sepals were shorter than the wild type, but there was no difference between the transgenic plants and the wild type in flowering time. These results suggest that PvVRN1 gene may be involved in the flower morphogenesis and other developmental processes of Phyllostachys phyllostachys. The results of this study may provide scientific basis for elucidating the flowering mechanism of bamboo.
【作者单位】：浙江农林大学亚热带森林培育国家重点实验室培育基地;
【基金】：国家自然基金(31270715;31000295) 国家留学基金国家级大学生创新创业训练计划项目(201410341021)
【分类号】：S795
【正文快照】： 由营养生长转向生殖生长是植物发育的一个重要过程。对双子叶模式植物拟南芥(Arabidopsisthaliana)的研究发现,有4条途径调控植物这一转变过程:光周期途径[1]、春化途径[2]、赤霉素(GA)途径[3]、自主途径[4]。VRN1基因是介导春化途径的一个重要基因,主要通过与VRN2基因共同维

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