施氮量对烤烟成熟期中部烟叶碳氮代谢及相关基因表达的影响

发布时间：2018-02-03 01:18

本文关键词： 氮肥烤烟细胞超微结构碳氮代谢基因表达　出处：《烟草科技》2017年08期 　论文类型：期刊论文

【摘要】：为探明不同施氮量处理对烤烟碳氮代谢的影响,以烤烟品种豫烟10号为材料进行试验,施氮量分别设置低氮(22.5 kg/hm~2)、中氮(26.25 kg/hm~2)和高氮(30 kg/hm~2)3个处理,研究烤烟成熟期中部烟叶细胞的超微结构、碳氮代谢产物的变化规律和碳氮代谢途径相关基因的表达模式。结果表明:随土壤氮肥施用量的增加,烟叶细胞中淀粉粒体积增大,烟叶总糖和还原糖含量降低,淀粉和总氮含量增加。碳代谢关键基因AGPase的表达量随烟叶的生长而增加,但增加量随施氮量的增加而逐渐减少;氮代谢关键基因NR的表达量则随施氮量的增加而下降。
[Abstract]:In order to find out the effect of different nitrogen application amount on carbon and nitrogen metabolism of flue-cured tobacco, the flue-cured tobacco variety Yuyan 10 was used as the material, and the nitrogen application rate was 22.5 kg / hm ~ (-2). The ultrastructure of tobacco cells in the middle of flue-cured tobacco was studied under three treatments of 26.25 kg 路hmm-2) and 30 kg / hmm-2). The changes of carbon and nitrogen metabolites and the expression pattern of genes related to carbon and nitrogen metabolism pathway. The results showed that the volume of starch grains in tobacco leaf cells increased with the increase of nitrogen fertilizer application in soil. The content of total sugar and reducing sugar decreased, and the content of starch and total nitrogen increased. The expression of AGPase, the key gene of carbon metabolism, increased with the growth of tobacco leaves, but the increase gradually decreased with the increase of nitrogen application. The expression of NR, the key gene of nitrogen metabolism, decreased with the increase of nitrogen application rate.
【作者单位】：河南农业大学烟草学院国家烟草栽培生理生化基地;
【基金】：中国烟草总公司特色优质烟叶开发重大专项“浓香型特色优质烟叶开发项目-浓香型特色优质烟叶形成的代谢与分子基础研究”(110200902045 TS-01)
【分类号】：S572
【正文快照】： 碳氮代谢是烟草生长发育的基本代谢,对烟草品质具有重要影响[1]。烟草碳氮代谢产物的合成受光照、温度、干旱、海拔高度及施肥水平等因素的影响,氮素是影响烟叶碳氮代谢产物合成的关键因素之一[2-7]。韩锦峰等[6]研究发现随着施氮量的增加,烤后烟叶中的总氮和烟碱含量呈增加

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