小麦品种‘武农148’和‘西农928’的抗叶锈病基因分析

发布时间：2018-02-03 05:33

本文关键词： 小麦叶锈菌基因推导成株抗病鉴定分子检测　出处：《河北农业大学学报》2017年02期 　论文类型：期刊论文

【摘要】：本研究选用9个小麦叶锈菌菌系接种36个已知基因载体品种(系)、‘武农148’和‘西农928’进行抗叶锈基因苗期推导分析,在2014—2015和2015—2016连续两年两点对其进行成株抗叶锈性鉴定并结合苗期抗叶锈基因推导与系谱分析;利用9个与已知抗病基因紧密连锁的特异性标记进行标记检测,从而得出供试材料的抗性和携带的抗病基因。结果表明,‘西农928’中可能携带抗病基因Lr30、Lr42和携带有未知微效抗病基因,所检测的2个品种均不含抗病基因Lr1、Lr9、Lr10、Lr19、Lr20、Lr24、Lr26、Lr34和Lr46。针对西农928中含有的抗叶锈病基因,可作为小麦抗锈病育种中的抗源材料。
[Abstract]:In this study, 9 lines of wheat leaf rust were inoculated with 36 known gene vector varieties (Wunong 148' and 'Xinong 928') to analyze the seedling stage of leaf rust resistance genes. The leaf rust resistance of adult plants was identified in 2014-2015 and 2015-2016 for two consecutive years, and combined with the derivation and pedigree analysis of leaf rust resistance genes at seedling stage. Nine specific markers closely linked to known resistance genes were used to detect the resistance of the tested materials and the disease-resistant genes carried by the tested materials. 'Xinong 928' may carry the disease resistance gene Lr30 Lr42 and the unknown microfunctional resistance gene. The two cultivars tested did not contain the disease resistance gene Lr1, Lr9, Lr10. Lr19, Lr20, Lr24, Lr26, Lr34 and Lr46. for the resistance genes contained in Xinong 928, they can be used as resistant materials in wheat breeding for resistance to rust.
【作者单位】：河北农业大学农学院/华北作物种质资源研究与利用教育部重点实验室;保定第三中学;
【基金】：河北省应用基础研究计划重点基础研究项目(11960145D)
【分类号】：S435.12
【正文快照】： 由叶锈菌(Puccinia tirticina)侵染引起的小麦叶锈病,是危害我国小麦产量的重要因素之一,短期内可导致大面积发病,严重时可造成产量损失达到40%[1]。近十几年来,在中国小麦主产区尤其是华北和黄淮流域发病甚重[2]。2012年在中国的甘肃、四川、陕西、河南和安徽等地发病严重,造

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