鸡源ADAR2基因克隆及其抗新城疫病毒作用研究

发布时间：2018-02-04 09:00

本文关键词： 鸡双链RNA特异性腺苷脱氨酶基因新城疫病毒 RNA编辑抗病毒作用　出处：《中国动物传染病学报》2017年02期 　论文类型：期刊论文

【摘要】：目前对家禽中双链RNA特异性腺苷脱氨酶(adenosine deaminase acting on double-stranded RNA,ADAR)的种类、组织分布及其功能尚不明了。本研究中以刀豆素、poly I:C以及新城疫病毒(Newcastle disease virus,NDV)作为刺激物诱导鸡胚成纤维细胞(chicken embryo fibroblast,CEF)中ADARs基因上调,扩增获得鸡ADAR2基因的mRNA。为了鉴定ADAR2的抗病毒作用,本研究构建了能够偶联有绿色荧光蛋白(green fluorescent protein,GFP)的ADAR2重组真核表达质粒,转染DF1细胞,结果显示在过量表达ADAR2的细胞中,NDV的增殖受到了显著抑制,表明ADAR2在NDV感染过程中发挥重要的抗病毒作用,这为进一步研究鸡RNA编辑酶对NDV RNA编辑奠定了基础。
[Abstract]:At present, adenosine deaminase acting on double-stranded RNA, a double-stranded RNA specific adenosine deaminase, was used in poultry. The species, tissue distribution and function of ADAR are unknown. Concanavalin is used in this study. Poly I: C and Newcastle disease virus. NDV was used as a stimulant to induce the up-regulation of ADARs gene in chicken embryo fibroblast cells. The mRNAs of chicken ADAR2 gene were amplified. In order to identify the antiviral effect of ADAR2. In this study, the recombinant eukaryotic expression plasmid of ADAR2 was constructed, which could couple the green fluorescent protein with green fluorescent protein (GFP). The results of transfection of DF1 cells showed that the proliferation of ADAR2 was significantly inhibited in the cells over-expressing ADAR2, indicating that ADAR2 played an important antiviral role in the process of NDV infection. This laid a foundation for further study on the editing of NDV RNA by chicken RNA editing enzyme.
【作者单位】：中国农业科学院上海兽医研究所;
【基金】：公益性行业(农业)科研专项经费(201303033) 国家自然科学基金(31101822,31530074)
【分类号】：S852.65
【正文快照】： 双链R N A特异性腺昔脱氨酶(a d e n o s i n edeaminase acting on double-stranded RNA,ADAR)基因家族广泛存在于从线虫到人的各种生物细胞中,因其所编码的RNA编辑酶可作用于特定双链RNA结构和m RNA前体,引起腺苷发生脱氨基变成肌苷(A→I)而得名,这一现象被命名为“RNA编辑

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