敦煌石室大宝胶囊对衰老大鼠血清白细胞介素-4、-10含量和海马区热休克蛋白86基因蛋白表达的影响

发布时间：2018-02-05 16:53

本文关键词： 白细胞介素- 白细胞介素- 热休克蛋白脑海马组织敦煌石室大宝胶囊衰老　出处：《中国老年学杂志》2017年16期 　论文类型：期刊论文

【摘要】：目的观察以健脾补肾-涤痰祛瘀法为指导的敦煌石室大宝胶囊(DHDB)对衰老模型大鼠血清白细胞介素(IL)-4、IL-10含量和海马区热休克蛋白(HSP)86基因和蛋白表达的影响。方法受试动物按随机数字表法分为空白组(n=10)和模型组(n=50)。采用D-半乳糖(0.125 g/kg)皮下注射建立衰老模型并筛选符合衰老模型的大鼠,按随机数字表法分为模型组、DHDB高、中、低剂量组(0.8、0.4、0.2 g/kg)和阳性对照组(0.4 g/kg吡拉西坦),经药物干预后分别测定各组大鼠血清IL-4、IL-10含量和海马区HSP86基因和蛋白表达水平。结果与空白组比较,模型组大鼠学习能力下降,血清IL-4含量显著降低、IL-10含量显著增高,海马区HSP86基因和蛋白表达水平显著降低(均P0.05);DHDB各剂量组大鼠学习能力提高,血清IL-4含量显著增高而IL-10含量显著降低,海马区HSP86基因和蛋白表达水平显著增高,且以高剂量DHDB作用为优(P0.05)。结论 DHDB具有提高衰老大鼠学习记忆能力,调节血清IL-4、IL-10含量正常分泌及促进海马区HSP86基因和蛋白表达的作用。
[Abstract]:Objective to observe the effect of DHDBs of Dunhuang Shizhuangdabao capsule (DHDBs) on serum interleukin IL-4 in aging model rats guided by the method of invigorating spleen and kidney and removing phlegm from phlegm and removing blood stasis. Effects of IL-10 content and heat shock protein (HSPN) 86 gene and protein expression in hippocampal region. Methods the tested animals were randomly divided into two groups: control group (n = 10) and model group (n = 50). The aging model was established by subcutaneous injection of D-galactose 0.125 g / kg and the aging model was selected. According to the method of random number table, the model group was divided into three groups: model group with high, medium and low doses of 0.8g / kg 0.40.2g / kg) and positive control group with 0.4 g / kg Piracetam). Serum IL-4 IL-10 level and HSP86 gene and protein expression level in hippocampus were measured after drug intervention. Results compared with the blank group, the learning ability of the model group decreased. The level of serum IL-4 decreased significantly, the level of IL-10 increased significantly, and the expression of HSP86 gene and protein in hippocampus decreased significantly (all P 0.05). The learning ability of rats in each dose group of DHDB was improved, the content of serum IL-4 was significantly increased, the content of IL-10 was significantly decreased, and the expression of HSP86 gene and protein was significantly increased in hippocampus. Conclusion DHDB can improve learning and memory ability and regulate serum IL-4 in aging rats. Normal secretion of IL-10 and promotion of HSP86 gene and protein expression in hippocampus.
【作者单位】：甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室;甘肃中医药大学甘肃省中药新产品创新工程重点实验室;
【基金】：敦煌医学与转化省部共建教育部重点实验室开放基金(No.DHYX1213-009) 甘肃省科技攻关计划项目(No.2GS064-A43-020-22) 兰州市科技局计划项目(No.06-2-87)
【分类号】：R285.5

【相似文献】