棉花黄萎病菌T-DNA插入突变体功能基因及生物学性状分析

发布时间：2018-02-08 10:25

本文关键词： 棉花黄萎病大丽轮枝菌 T-DNA插入突变生物学性状基因功能　出处：《重庆师范大学学报(自然科学版)》2017年03期 　论文类型：期刊论文

【摘要】：【目的】分析棉花黄萎病致病菌相关基因的功能揭示其中致病机制。【方法】通过ATMT法构建了大丽轮枝菌(Verticillium dahliae)650个T-DNA插入突变体。利用hiTAIL-PCR技术获得17株T-DNA插入体侧翼基因序列,通过blast比对分析表明,其中16个基因序列编码酶或蛋白。【结果】突变体的生物学性状与野生型菌株Vd991相比,出现黄色菌丝型2株,中间菌丝型6株;部分突变体在生长速率(7株)、产孢量(14株)和粗毒素产量(4株)上都发生了具有统计学意义的变化(p0.05);致病力测定表明,9株突变体的致病力较野生型有所降低,在p0.05水平具有统计学意义。【结论】该研究为大丽轮枝菌致病基因的筛选和鉴定提供了理论基础。
[Abstract]:[objective] to analyze the function of genes associated with Verticillium dahlia in cotton to reveal the pathogenic mechanism. [methods] 650 T-DNA insertion mutants of Verticillium dahlia were constructed by ATMT method, and 17 T-DNA inserts were obtained by hiTAIL-PCR technique. According to blast comparison analysis, 16 of them encode enzyme or protein. [results] compared with wild-type strain Vd991, two yellow mycelia and six intermediate mycelia were found in the mutant. The pathogenicity of 9 mutants was lower than that of wild type, and there were significant changes in growth rate (7 strains, sporulation: 14 strains) and crude toxin yield (4 strains) in some mutants (P < 0.05), the pathogenicity test showed that the pathogenicity of 9 mutants was lower than that of wild-type mutants, and the pathogenicity of 9 mutants was lower than that of wild-type mutants. There was statistical significance at p0.05 level. [conclusion] this study provides a theoretical basis for screening and identification of pathogenic genes of Trichoderma grandis.
【作者单位】：重庆师范大学植物抗病实验室重庆市植物逆境生物学重点实验室;
【基金】：国家自然科学基金(No.40103112) 重庆市教委项目(No.KJ1500324)
【分类号】：S435.62

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