ACAT1基因干扰对人结肠癌细胞增殖及侵袭转移的影响

发布时间：2018-02-08 16:27

本文关键词： 结肠肿瘤酰基辅酶A-胆固醇酰基转移酶小干扰RNA　出处：《实用医学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:利用小干扰RNA(si RNA)技术干扰酰基辅酶A-胆固醇酰基转移酶1(ACAT1)基因在人结肠癌HT29细胞中的表达,观察ACAT1基因表达下降对于人结肠癌HT29细胞增殖和侵袭迁移能力的影响,了解ACAT1在结肠癌发生发展中的作用。方法:通过脂质体Hiper Fect将ACAT1 si RNA转染至人结肠癌HT29细胞中干扰ACAT1基因的表达,利用Real time PCR检测转染前后ACAT1基因表达的变化,利用ACAT1基因干扰前后的HT29细胞,采用CFSE染色法检测HT29细胞增殖能力的变化;Transwell小室法检测HT29细胞侵袭和迁移能力的变化。结果:ACAT1 si RNA转染HT29细胞后ACAT1 m RNA的表达明显下降,ACAT1 si RNA转染后的HT29细胞与转染前比较,增殖及侵袭转移的能力均受到明显抑制,差异有统计学意义(P0.05)。结论:ACAT1基因干扰使HT29细胞的增殖减慢,侵袭迁移能力降低,为结肠癌治疗提供新的靶点。
[Abstract]:Aim: to investigate the effect of the decrease of ACAT1 gene expression on the proliferation, invasion and migration of human colon cancer HT29 cells by interfering with the expression of acyl coenzyme A- cholesterol acyltransferase 1 ActaT1 gene in human colon cancer HT29 cells by using small interfering RNA(si RNAs. Methods: ACAT1 si RNA was transfected into human colon cancer HT29 cells by liposome Hiper Fect to interfere with the expression of ACAT1 gene. Real time PCR was used to detect the changes of ACAT1 gene expression before and after transfection. Using ACAT1 gene to interfere with HT29 cells, The changes of proliferation ability of HT29 cells were detected by CFSE staining and the changes of invasion and migration of HT29 cells were detected by Transwell chamber assay. Results the expression of ACAT1 m RNA in HT29 cells transfected with HT29 cells was significantly decreased after transfection of HT29 cells by RNA. The expression of ACAT1 m RNA in HT29 cells after transfection of HT29 cells was significantly lower than that in HT29 cells after transfection with ACAT1si RNA. The ability of proliferation and invasion and metastasis were significantly inhibited, and the difference was statistically significant (P 0.05). Conclusion the interference of the HT29 gene can slow down the proliferation and decrease the ability of invasion and migration of HT29 cells, which provides a new target for colon cancer therapy.
【作者单位】：武汉大学人民医院肿瘤科;武汉大学人民医院胃肠外科;
【基金】：中央高校基本科研业务费专项基金资助项目(编号:2042014kf0149) 湖北省自然科学基金资助项目(编号:2012FKC143)
【分类号】：R735.35

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