HPV16基因整合与FHIT蛋白缺失在宫颈癌变中的作用

发布时间：2018-02-09 00:43

本文关键词： 人乳头状瘤病毒脆性组氨酸三联体整合宫颈上皮内瘤样病变宫颈癌　出处：《中国妇产科临床杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨HPV16的基因型整合状态及FHIT基因缺失在宫颈癌发生发展中的作用及相关性。方法选取44例宫颈癌、58例宫颈上皮内瘤样病变(CIN)和20例宫颈正常组织的患者,免疫组化检测宫颈FHIT蛋白的表达;并用多重PCR法检测HPV16 E2/E7表达。结果 122例单一HPV16阳性标本HPV16总整合率为78.69%,在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中整合率分别为60.00%、66.67%、75.00%、86.96%和88.63%,随病变加重,整合率增强,组间差异有统计学意义(P0.05);FHIT蛋白的总缺失率为43.44%,FHIT蛋白在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中的缺失率分别为15.00%、20.00%、25.00%、43.48%和72.73%。FHIT蛋白的缺失率,随病变加重,缺失率增高,组间差异有统计学意义(P0.05)。FHIT蛋白缺失在不同HPV16整合时不同,差异有统计学意义(P0.05)。结论 HPV16基因整合和FHIT蛋白缺失在宫颈癌的发生、发展中起重要作用,HPV16基因整合可能通过诱导FHIT基因低表达从而促使宫颈癌发生发展。
[Abstract]:Objective to investigate the role of HPV16 genotype integration and FHIT gene deletion in the occurrence and development of cervical cancer. Methods 44 patients with cervical carcinoma and 58 patients with cervical intraepithelial neoplasia (cin) and 20 patients with normal cervical tissue were selected. The expression of FHIT protein was detected by immunohistochemistry, and the expression of HPV16 E2 / E7 was detected by multiple PCR method. Results the total integration rate of HPV16 in 122 single HPV16 positive specimens was 78.699.The integration rates in normal cervical tissues were 60.0066.67% and 75.0086.96%, respectively, and 88.63% in cervical cancer. The total deletion rate of FHIT protein was 43.44% in normal cervical tissue and 15.00% in normal cervical tissue. The deletion rate of FHIT protein in normal cervical tissue was 15.00% and 20.00% respectively, and the deletion rate of FHIT protein was 72.73.FHIT protein was 72.73.FHIT protein deletion rate was higher than that of normal cervix tissue, and the total deletion rate of FHIT protein was 43.48% and 72.73.FHIT protein deletion rate was higher than that in normal cervical tissue. There was significant difference between the two groups in the deletion of P0.05N. FHIT protein in different HPV16 integration, and the difference was statistically significant. Conclusion the HPV16 gene integration and FHIT protein deletion may be involved in the occurrence of cervical cancer. HPV16 gene integration may promote the development of cervical cancer by inducing low expression of FHIT gene.
【作者单位】：湖北省武汉市第五医院;广西柳州市人民医院妇产科;华中科技大学同济医学院附属同济医院妇产科;

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