体内Pig-a基因突变试验检测亚硒酸钠的致突变作用

发布时间：2018-02-09 20:34

本文关键词： 亚硒酸钠 Pig-a基因致突变作用　出处：《现代预防医学》2017年15期 　论文类型：期刊论文

【摘要】：目的采用改良后的基于流式细胞术的大鼠体内Pig-a基因突变试验方法来检测和评价亚硒酸钠的体内致突变作用,为研究亚硒酸钠的遗传毒性和致癌性提供一定的科学依据。方法将30只雄性SD大鼠分别连续3天灌胃给予0.625(低)、1.25(中)和2.5 mg/kg.bw(高)亚硒酸钠,同时连续3天给予40 mg/kg.bw N-乙基-N-亚硝基脲(ENU)作为阳性对照。在给药前和首次给药后15天、30天和45天进行尾静脉采血,利用流式细胞仪检测样本中红细胞突变率。结果随着染毒时间的延长及染毒剂量增大,RBCCD59-率和RETCD59-率均呈上升趋势。15天时,1.25 mg/kg.bw组RBCCD59-率高于空白对照组,差异有统计学意义(P0.05),30天时,1.25、2.5 mg/kg.bw组RBCCD59-率高于空白对照组,差异有统计学意义(P0.05)。45天时,亚硒酸钠各组RBCCD59-率均高于空白对照组,差异有统计学意义(P0.05);30天和45天时,亚硒酸钠高剂量组RETCD59-率高于空白对照组,差异有统计学意义(P0.05)。同时随时间延长,出现大鼠体重下降的趋势,且中、高剂量组均出现了死亡的现象。结论亚硒酸钠在一定剂量下具有弱致突变作用,同时还具有一般毒性作用。
[Abstract]:Objective to detect and evaluate the mutagenicity of sodium selenite in vivo by a modified flow cytometry based Pig-a gene mutation assay in rats. Methods 30 male Sprague-Dawley rats were gavaged with 0.625 (middle) and 2.5 mg / kg 路bwite sodium selenite for 3 days, respectively, for the purpose of studying the genotoxicity and carcinogenicity of sodium selenite. At the same time, 40 mg/kg.bw N- ethyl-N- nitrosourea (ENUN) was given for 3 consecutive days as a positive control. The blood samples were collected from caudal vein before administration and 15 days and 45 days after first administration. Results the RBC CD59- rate and RETCD59- rate in 1.25 mg/kg.bw group were higher than those in the blank control group with the prolongation of exposure time and the increase of the dose of RBC CD59- at 15 days after exposure, the RBC CD59- rate in the sample was detected by flow cytometry, and the RBC CD59- rate and RETCD59- rate in 1.25 mg/kg.bw group were higher than those in the control group. The RBCCD59- rate of mg/kg.bw group was significantly higher than that of the blank control group at 30 days after 30 days, and the difference was significant at P0.05 and 45 days, the RBCCD59- rate of sodium selenite group was higher than that of the blank control group, and the difference was statistically significant at 30 and 45 days, respectively. The RETCD59- rate in the high dose sodium selenite group was higher than that in the blank control group, and the difference was statistically significant (P 0.05). Conclusion Sodium selenite has weak mutagenicity and general toxicity in a certain dose.
【作者单位】：四川大学华西公共卫生学院(华西第四医院)四川省食品安全监测与风险评估重点实验室;
【基金】：国家自然科学基金(NO.004040541058) 四川大学青年教师科研启动基金(NO.2015SCU11010)
【分类号】：R99

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