甘蔗胁迫诱导表达基因ScCBF1的功能分析

发布时间：2018-02-09 21:08

本文关键词： 甘蔗转录因子荧光定量PCR 胁迫瞬时表达　出处：《福建农林大学学报(自然科学版)》2017年02期 　论文类型：期刊论文

【摘要】：以甘蔗苗为材料研究了甘蔗CBF1转录激活因子ScCBF1在水杨酸(SA)、茉莉酸甲酯(Me JA)以及重金属镉(Cd Cl_2)和铜(Cu Cl_2)胁迫下的表达情况;将ScCBF1的原核表达载体p GEX-6P-1-ScCBF1转化大肠杆菌(Escherichia coli)Rosetta(DE3),检测其在NaCl、PEG8000胁迫下的生长情况.结果表明:ScCBF1在Me JA、SA、Cd Cl_2以及Cu Cl_2逆境胁迫下表达下调;在500 mmol·L-1NaCl胁迫下,含有重组质粒的细胞生长速度显著减缓,不同浓度NaCl胁迫下的平板胁迫结果进一步说明了ScCBF1基因不具耐盐性;在15%PEG8000胁迫下,含有重组质粒细胞的生长速度明显高于对照,不同浓度PEG8000胁迫下的平板胁迫结果说明了ScCBF1基因在应答干旱胁迫中具有抗逆性.构建了ScCBF1的植物表达载体p CAMBIA1301-ScCBF1并通过农杆菌侵染在本氏烟叶片中瞬时表达,在4℃低温胁迫下,T0代烟草植株长势显著优于对照.
[Abstract]:Study on the materials of sugarcane CBF1 transcription factor ScCBF1 in sugarcane seedlings by salicylic acid (SA), methyl jasmonate (Me JA) and cadmium (Cd Cl_2) and copper (Cu Cl_2) expression of stress; the ScCBF1 prokaryotic expression vector p and transformed into E.coli GEX-6P-1-ScCBF1 (Escherichia coli Rosetta (DE3)), detected by NaCl, the growth under PEG8000 stress. The results show that ScCBF1 in Me JA, SA, Cd Cl_2 and Cu Cl_2 stress expression; in 500 mmol under the stress of L-1NaCl, the recombinant plasmid containing the cell growth rate significantly slowed down, the plate under different concentration of NaCl stress results the ScCBF1 gene has no salt tolerance; under 15%PEG8000 stress, the growth rate of recombinant plasmid containing cells was significantly higher than the control panel, under different concentration of PEG8000 stress results show that with the ScCBF1 gene in response to drought stress The plant expression vector p CAMBIA1301-ScCBF1 of ScCBF1 was constructed and transiently expressed in tobacco leaves by Agrobacterium infection. The growth of T0 generation plants was significantly better than that of control under 4 chilling stress.

【作者单位】：福建农林大学/农业部福建甘蔗生物学与遗传改良重点实验室;
【基金】：国家高技术研究发展计划863计划(2013AA102604) 国家自然科学基金(31371688,31571732) 国家现代农业产业技术体系(CARS-20-1-1)
【分类号】：S566.1

【相似文献】