水貂源致病性大肠杆菌生物被膜形成能力、耐药性及毒力岛基因检测与分析

发布时间：2018-02-09 23:24

本文关键词： 致病性大肠杆菌水貂源生物被膜耐药性毒力岛　出处：《中国预防兽医学报》2017年05期 　论文类型：期刊论文

【摘要】：为探索水貂肠道致病性大肠杆菌(E.coli)生物被膜(BF)形成能力、耐药性、毒力岛基因分布及其之间可能存在的相关性,本研究对收集的临床分离鉴定的54株致病性E.coli,采用结晶紫染色定量法测定体外BF形成能力,K-B法药敏试验检测对10种常见抗菌药物的敏感性,PCR检测毒力岛代表基因。结果显示,54株E.coli BF形成阳性率为90.7%,以强阳性为主;分离株对氨苄西林、环丙沙星、青霉素和四环素的耐药率分别为100%、97.5%、92.5%和90%,对多黏菌素B的耐药率最低为17.5%,多数菌株呈现多重耐药,其中对6种以上抗菌药物耐药的占92.6%。毒力岛基因irp2和eae阳性率均为7.4%,未检测到SGI基因。这些分离株普遍具有较强BF形成能力,并呈现多重耐药性,BF形成能力与菌株对呋喃妥因、环丙沙星及氟苯尼考的耐药性呈正相关,但未发现和毒力基因分布率存在明显相关性。
[Abstract]:In order to explore the ability of formation, drug resistance, gene distribution of virulence island and the possible correlation between the biofilm of E. coli in mink, the ability of BFF formation, drug resistance and the distribution of virulence island gene in mink were studied. In this study, 54 strains of pathogenicity E.coli collected from clinical isolation and identification were detected by crystal violet staining quantitative method for BF formation ability in vitro. K-B method was used to detect susceptibility to 10 common antimicrobial agents. PCR was used to detect virulence island representative gene. The results showed that the positive rate of E. coli BF in 54 strains was 90.7, mainly strong positive. The resistant rates of ampicillin, ciprofloxacin, penicillin and tetracycline to ampicillin, ciprofloxacin, penicillin and tetracycline were 92.5% and 90%, respectively. The positive rates of virulence island gene irp2 and eae were 7.4and no SGI gene was detected. These isolates generally had strong BF forming ability and showed multidrug resistance and furantoin. The drug resistance of ciprofloxacin and florfenicol was positively correlated, but there was no significant correlation with virulence gene distribution.
【作者单位】：东北农业大学动物医学学院;哈药集团生物疫苗有限公司;中国农业科学院哈尔滨兽医研究所;
【基金】：东北农业大学大学生SIPT项目(20160224061) 兽医生物技术国家重点实验室开放课题资助项目(SKLVBF201611)
【分类号】：S852.61

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