基于RNA-Seq绒山羊皮肤两种毛囊差异表达基因的分析

发布时间：2018-02-12 11:25

本文关键词： 绒山羊毛囊转录组 RNA-测序　出处：《家畜生态学报》2017年11期 　论文类型：期刊论文

【摘要】：为了研究绒山羊皮肤初级毛囊和次级毛囊之间的表达基因模式的差异,筛选影响毛囊发育的重要基因,对6只内蒙古绒山羊阿尔巴斯型个体进行RNA-seq测序,利用生物信息学方法对转录组数据进行分析,在测序结果质量控制后的基础上,对差异表达基因进行筛选、功能注释和富集分析。共筛选到617个在初级毛囊和次级毛囊中差异表达的基因,其中,上调表达基因297个,下调表达基因320个。GO功能注释结果显示,上调表达基因主要行使电子传递链、呼吸链电子传递、线粒体电子传递、基因表达等生物学功能;下调表达基因主要行使细胞吞噬、蛋白质复性、中性粒细胞聚集、细菌真菌防御反应等生物学功能。利用KEGG数据库作为参考,这些基因主要参与半胱氨酸和蛋氨酸代谢、RNA聚合、RNA可变剪切、底物磷酸化等通路。PPP2R2A和CDK2AP1基因可能在皮肤毛囊发育中发挥重要作用,以上结果为今后深入研究皮肤毛囊发育相关基因及功能提供了依据。
[Abstract]:In order to study the difference of gene expression patterns between primary hair follicles and secondary hair follicles of cashmere goats, and to screen the important genes affecting the development of hair follicles, RNA-seq sequencing was carried out in 6 individuals of Albanian type in Inner Mongolia Cashmere Goat (Inner Mongolia Cashmere Goat). The transcriptome data were analyzed by bioinformatics, and the differentially expressed genes were screened on the basis of quality control of sequencing results. Functional annotation and enrichment analysis. A total of 617 differentially expressed genes were screened in primary hair follicles and secondary follicles, of which 297 genes were up-regulated and 320 down-regulated genes. The up-regulated genes mainly perform the biological functions of electron transfer chain, respiratory chain electron transport, mitochondrial electron transport, gene expression, etc. The down-regulated genes mainly perform phagocytosis, protein renaturation, neutrophil aggregation, etc. Using the KEGG database as a reference, these genes are mainly involved in the metabolism of cysteine and methionine. The phosphorylation of substrates. PPP2R2A and CDK2AP1 genes may play an important role in the development of skin hair follicles. These results provide a basis for the further study of the genes and functions related to the development of skin hair follicles.
【作者单位】：内蒙古农业大学动物科学学院;动物遗传育种与繁殖自治区重点实验室;农业部肉羊遗传育种重点实验室;
【基金】：国家绒毛用羊现代农业产业技术体系项目(CARS-39-06) 国家自然科学基金(31660639)
【分类号】：S827

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