pH法防止转基因蓝藻向环境释放

发布时间：2018-02-14 13:16

  本文关键词： pH 杀藻率 转基因蓝藻 环境释放 重组药物安全性 响应面法 重组药物制备　出处：《生物技术》2017年06期 　论文类型：期刊论文

【摘要】：[目的]防止转基因蓝藻(Anabaena sp.PPC 7120)在制备对虾抗白斑综合征病毒口服药过程中向环境释放,保证制药安全性。[方法]用不同pH处理转基因蓝藻,并在不同时间、温度和光照下观察效果,并用响应面法优化处理条件。[结果]最佳处理条件为:pH≤2.5、t≥50 min、T≥30℃或pH≥12、t≥120 min、T≥30℃,杀藻率为100%。两个优化组合分别为:pH 2.12、温度29.96℃、时间33.9 min或pH 12.33、温度31.75℃、时间96.8 min。pH处理后的转基因蓝藻在平板上不能再继续生长。[结论]pH法对转基因蓝藻的杀藻率可达100%,有效防止了转基因蓝藻在制备对虾口服剂过程中发生环境释放。
[Abstract]:[objective] to prevent the release into the environment of Anabaena sp.PPC 7120) during the preparation of antileukoplakia syndrome virus oral drug from Penaeus Monodon, and to ensure the pharmaceutical safety. [methods] transgenic cyanobacteria were treated with different pH and at different times. The effect was observed under temperature and light, and the response surface method was used to optimize the treatment conditions. [results] the optimal treatment conditions were as follows: 1: ph 鈮，

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