棉花不同茸毛性状近等基因系的选育与生理生化特性研究

发布时间：2018-02-16 17:22

本文关键词： 近等基因系茸毛性状叶绿素含量苯丙氨酸解氨酶单宁　出处：《华北农学报》2017年03期 　论文类型：期刊论文

【摘要】：为了培育具有抗病虫优良性状的棉花新品种,并对棉花多茸毛基因进行定位克隆,从棉花三元杂种后代中选育了绿叶无茸毛和绿叶多茸毛近等基因系。以该近等基因系为供试材料,测定了具有不同茸毛性状的棉株间光合速率、叶绿素含量、苯丙氨酸解氨酶(PAL)活性以及单宁含量的变化,通过光合生理与相关生化指标测定相结合,初步探讨棉花不同茸毛性状近等基因系材料间生理生化特性的差异,为今后选育具有优良性状的棉花新品种提供依据。结果表明,无茸毛与多茸毛棉花近等基因系的叶绿素a、b及总量的动态变化趋势基本一致,蕾期较高,花期、铃期降低;净光合速率变化趋势一致,二者均呈先上升后下降再上升的趋势,在铃期达到了顶峰。因此,光合速率与叶绿素含量变化趋势在近等基因系材料间差异不明显。无茸毛和多茸毛棉花纤维和种皮单宁含量变化具有相同的趋势,均为先升高再降低,纤维中的PAL活性多茸毛棉株逐渐下降,无茸毛棉株下降后再升高,而种皮中PAL活性上升至最高值后保值不变。PAL活性与单宁含量在材料间差异较大,绿叶多茸毛棉株高于绿叶无茸毛棉株。表明PAL活性和单宁含量与茸毛性状存在一定的相关关系。
[Abstract]:In order to develop new cotton varieties with excellent pest resistance traits, and to locate and clone the polyfurry gene in cotton, The green leaf hairless and green leaf hairy near isogenic lines were selected from the hybrid progenies of cotton. The photosynthesis rate and chlorophyll content of cotton plants with different fluffy characters were measured by using this near isogenic line as the test material. The changes of phenylalanine ammonia-lyase (pal) activity and tannin content in cotton were studied. The physiological and biochemical characteristics of nearly isogenic lines with different hairy traits in cotton were preliminarily studied by combining photosynthesis physiology with related biochemical indexes. The results showed that the dynamic change trend of chlorophyll a b and total amount of near isogenic lines was basically the same, the bud stage was higher, the flowering stage and boll stage were lower than that of non-hairy cotton lines, the results showed that the dynamic change trend of chlorophyll a b and the total amount of chlorophyll a b in the near isogenic lines of non-hairy and multi-hairy cotton was basically the same. The change trend of net photosynthetic rate was the same. Both of them showed the trend of rising first, then decreasing and then rising, and reached the peak at boll stage. There was no significant difference in photosynthetic rate and chlorophyll content between near isogenic lines. The changes of fiber and tannin content in non-hairy and polyfurry cotton had the same trend, which increased first and then decreased. The activity of PAL in the fiber decreased gradually, but the activity of PAL in the seed coat increased after the decrease, while the activity of pal and the content of tannin in the seed coat increased to the highest value, and there was a great difference between the two materials. The results showed that the activity of PAL and the content of tannin had some correlation with hairy characters.
【作者单位】：山西农业大学农学院;
【基金】：棉花生物学国家重点实验室开放课题(CB2015A20) 山西省科技攻关项目(20140311004-3;20130311004-3)
【分类号】：S562

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