EBV转化B淋巴细胞的差异基因筛选

发布时间：2018-02-21 12:36

本文关键词： EB病毒 B细胞转化基因芯片差异基因　出处：《广东医学》2017年19期 　论文类型：期刊论文

【摘要】：目的通过全基因组芯片筛选正常人外周血淋巴细胞(PBLs)和EB病毒(EBV)转化B淋巴细胞株CGM1中的差异基因,探讨EBV转化B淋巴细胞的机制。方法分离培养正常人外周血淋巴细胞(PBLs),通过原位杂交检测PBLs和CGM1细胞中EBER表达情况;提取2株细胞RNA,利用人全基因组寡核苷酸基因表达谱芯片筛选2株细胞之间差异表达基因,并通过生物信息学对差异表达基因进行GO pathways和KEGG pathways分析。结果 EBER原位杂交结果显示PBLs EBER(-),CGM1 EBER(+);CGM1细胞与PBLs之间差异有统计学意义的基因有1 587个,其中上调基因897个,下调基因690个,这些基因主要涉及P53、PI3K-Akt、Ras、MAPK、Tolllike receptor、WNT、TNF和JAK-STAT等信号通路,与细胞周期调节,B细胞增殖、活化、分化和细胞因子产生,DNA复制、损伤修复,病毒与宿主相互作用及病毒致癌作用等相关。结论 EBV通过细胞周期调节、细胞活化与分化、DNA复制和损伤修复、病毒与宿主相互作用等多个环节参与B淋巴细胞转化。
[Abstract]:Objective to screen the differentially expressed genes in CGM1 of B lymphocyte strain transformed by EBV and PBLsof normal human peripheral blood lymphocytes (PBLs) and EB virus (EBV) by whole genome microarray. Methods the expression of EBER in PBLs and CGM1 cells was detected by in situ hybridization. Two cell RNAs were extracted and differentially expressed genes were screened by using the whole genome oligonucleotide gene expression microarray. By bioinformatics, go pathways and KEGG pathways were used to analyze the differentially expressed genes. Results EBER in situ hybridization showed that there were 1 587 genes with significant difference between PBLs EBER-CGM 1 cells and PBLs, among which 897 genes were up-regulated. There are 690 down-regulated genes. These genes are mainly involved in the signaling pathways such as P53, PI3K-AktRas MAPK, Tolllike receptor, TNF- and JAK-STAT, and regulate cell cycle, such as proliferation, activation, differentiation and cytokine production of DNA replication, damage and repair. Conclusion EBV is involved in B lymphocyte transformation through cell cycle regulation, cell activation and differentiation, replication and repair of DNA damage, and virus / host interaction.
【作者单位】：海南医学院第一附属医院病理科;
【基金】：海南省自然科学基金立项项目(编号:812187、814288) 海南省高等学校科学研究项目(编号:Hjkj2012-30) 海南医学院附属医院中青年科研培育基金立项课题(编号:HYFYPY201401)
【分类号】：R392

【相似文献】