豆梨NAC转录因子基因PcNAC1的克

发布时间：2018-02-22 18:17

本文关键词： 豆梨黑斑病 NAC转录因子亚细胞定位防卫反应　出处：《园艺学报》2017年07期 　论文类型：期刊论文

【摘要】：根据豆梨(Pyrus calleryana Dence)接种黑斑病菌后的转录组测序结果,筛选出一个上调表达的NAC转录因子基因。从豆梨中获得了该基因的全长片段,并将其命名为PcNAC1。该基因的开放阅读框为792 bp,编码263个氨基酸。将PcNAC1蛋白序列与其他物种蛋白序列进行对比,并构建系统进化树后发现,其与小麦TaNAC4及拟南芥ATAF1具有较高同源性。通过实时荧光定量分析PcNAC1在豆梨不同组中的表达发现,PcNAC1在茎、叶和根中表达量较高,在花和果中表达量较低。构建了亚细胞定位载体,瞬时侵染本氏烟,通过激光共聚焦显微镜观察融合蛋白在细胞内的分布情况,发现该基因行使功能的主要区域定位在细胞核。进一步在本氏烟中瞬时表达PcNAC1,接种烟草疫霉病菌后发现PcNAC1可以通过调控植物激素通路防卫反应基因的表达来增强植物的抗病性。
[Abstract]:According to the results of transcriptome sequencing after inoculating Pyrus calleryana Dence, a up-regulated NAC transcription factor gene was screened, and the full-length fragment of NAC transcription factor gene was obtained from soybean pear. The gene was named PcNAC1.The open reading frame of the gene was 792bp, encoding 263 amino acids. The sequence of PcNAC1 protein was compared with that of other species, and the phylogenetic tree was constructed. It has high homology with wheat TaNAC4 and Arabidopsis thaliana ATAF1. By real-time fluorescence quantitative analysis of PcNAC1 expression in different groups of pears, it was found that PcNAC1 expression was higher in stem, leaf and root, but lower in flower and fruit. Subcellular localization vector was constructed. The distribution of the fusion protein in the cells was observed by confocal laser microscopy. It was found that the main functional region of the gene was located in the nucleus. Furthermore, PcNAC1 was expressed in tobacco. After inoculation with Phytophthora tabacum, it was found that PcNAC1 could enhance plant disease resistance by regulating the expression of phytohormone pathway defense response gene.
【作者单位】：南京农业大学园艺学院;江苏省农业科学院果树研究所;江苏省农业科学院生物技术研究所;
【分类号】：S436.612

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