子宫腺肌病在位内膜差异基因表达及信号转导网络构建

发布时间：2018-02-23 23:20

本文关键词： 子宫腺肌病在位内膜信使RNA 信号转导网络　出处：《现代妇产科进展》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨信使RNA(mRNA)在子宫腺肌病在位内膜组织中的差异表达情况,并构建差异基因间的信号转导网络。方法:收集4例子宫腺肌病患者的在位内膜组织及4例正常内膜组织。采用基因芯片检测mRNA表达,对差异表达的mRNA进行信号通路分析,得到差异基因参与的具有显著性、低误判率、靶向性的信号通路。基于KEGG数据库构建基因间的信号转导网络并得到网络中的关键基因。结果:子宫腺肌病在位内膜与对照内膜相比,有612个差异表达的mRNA,其中下调的有414个,上调的有198个。上调差异基因参与的显著性信号转导通路共40项,包括MAPK信号通路、局部黏附、黏附连接、PI3K-Akt信号通路等;下调差异基因参与的显著性信号转导通路共39项,包括DNA复制、细胞周期、细胞因子、细胞因子受体相互作用等。成功构建信号转导网络,得到网络中关键基因包括HDAC2和ACTN4等。结论:与正常内膜相比,子宫腺肌病在位内膜的mRNA存在明显的差异表达,可能与子宫腺肌病的发生发展密切相关。
[Abstract]:Objective: to investigate the differential expression of messenger RNA-mRNAs in eutopic endometrium of adenomyosis. Methods: the eutopic endometrial tissues of 4 patients with adenomyosis and the normal endometrium were collected. The expression of mRNA was detected by gene microarray and the signal pathway of differentially expressed mRNA was analyzed. The results showed that there were significant differences in gene involvement and low misjudgment rate. Signal transduction network between genes was constructed based on KEGG database and key genes in the network were obtained. Results: there were 612 differentially expressed mRNAs in eutopic endometrium of adenomyosis compared with control endometrium, of which 414 were down-regulated. There were 198 up-regulated genes involved in 40 significant signal transduction pathways, including MAPK signaling pathway, local adhesion, adhesion to PI3K-Akt signal pathway, and down-regulation of 39 significant signal transduction pathways involving differential genes. Including DNA replication, cell cycle, cytokine, cytokine receptor interaction, etc. Signal transduction networks were successfully constructed, and the key genes in the network including HDAC2 and ACTN4 were obtained. Conclusion: compared with normal endometrium, the key genes in the network are HDAC2 and ACTN4. The expression of mRNA in eutopic endometrium of adenomyosis may be closely related to the occurrence and development of adenomyosis.
【作者单位】：中南大学湘雅三医院妇产科;
【分类号】：R711.71

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