石榴PgUFGT基因克隆及表达分析

发布时间：2018-02-24 06:39

  本文关键词： 石榴 UFGT 基因克隆 表达分析　出处：《西北植物学报》2017年04期 　论文类型：期刊论文

【摘要】：利用RT-PCR和RACE方法,从石榴(Punica granatum L.)果皮中克隆到一个类黄酮糖基转移酶(UFGT)基因(PgUFGT)全长cDNA序列(GenBank登录号为KF841620)。PgUFGT基因编码区1 476bp,编码491个氨基酸。PgUFGT蛋白具有保守PSPG基序、UDP-糖基转移酶家族结构域和UDP-葡萄糖醛酸基/葡萄糖基转移酶保守域(UDPGT),与其他植物UFGT蛋白一致性较高;系统进化树分析结果表明,PgUFGT属于类黄酮3-O-糖基转移酶类。荧光定量qRT-PCR结果表明,PgUFGT基因在‘红宝石’和‘水晶甜’2个石榴品种的发育期内具有不同的表达模式,PgUFGT在‘红宝石’石榴中有2个转录表达高峰,而在‘水晶甜’石榴中仅有1个表达高峰,表明PgUFGT可能在2个石榴品种中具有不同的催化作用。该研究结果为进一步研究石榴果实色泽形成的分子机制奠定了基础。
[Abstract]:Using RT-PCR and RACE methods, A full-length cDNA sequence of flavonoid glycosyltransferase (granatum granatum) gene was cloned from the pericarp of Punica granatum L.). GenBank accession number is 1 476 BP, encoding 491 amino acids. PgUFGT protein has conserved PSPG motif and UDP- glycosyltransferase family knot. The conserved domain of UDP-glucuronyl / glucosyltransferase (UDP-glucuronyl / glucosyltransferase) conserved with UFGT protein in other plants. Phylogenetic tree analysis showed that PgUFGT belonged to flavonoid 3-Oglycosyltransferase. Fluorescence quantitative qRT-PCR showed that PgUFGT gene had different expression patterns during the development period of 'ruby' and 'crystal sweet' pomegranate. There were two peaks of transcription expression in 'ruby' pomegranate. However, there was only one peak expression in 'crystal sweet' pomegranate, indicating that PgUFGT may have different catalytic effect in two pomegranate varieties. The results laid a foundation for further study on the molecular mechanism of fruit color formation of pomegranate.
【作者单位】： 南京林业大学南方现代林业协同创新中心;南京林业大学林学院;山东省果树研究所;
【基金】：国家自然科学基金项目(31272143) 南京林业大学高层次人才科研启动基金(GXL2014070) 江苏省高校优势学科建设工程项目(PAPD)
【分类号】：Q943.2;S665.4
，

本文编号：1529260