牙龈卟啉单胞菌唾液酸酶基因缺失影响其感染上皮细胞后炎症相关microRNA的表达

发布时间：2018-02-24 17:44

本文关键词： 牙龈卟啉单胞菌W PG 牙龈上皮细胞小RNA　出处：《口腔医学研究》2017年02期 　论文类型：期刊论文

【摘要】：目的:检测牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)唾液酸酶基因(PG0352)缺失是否影响其感染上皮细胞后对上皮细胞炎症相关小RNA的调控。方法:厌氧培养P.gingivalis W83及其唾液酸酶基因突变株(△PG0352),以MOI为100∶1的比例分别与永生化牙龈上皮细胞epi4进行共培养,PCR array的方法检测88个与炎症相关的小RNA的表达。结果:△PG0352组epi4的hsa-miR-9、hsa-miR-23a、hsa-miR-23b和hsa-miR-410的表达分别是P.gingivalis W83组的4.3387、3.3978、4.7930和4.3137倍。结论:与P.gingivalis W83野生株相比,唾液酸酶基因缺陷株对牙龈上皮细胞炎症相关小RNA的表达有不同的调控作用。
[Abstract]:Objective: to determine whether the deletion of porphyromonas gingivalis-P.gingivalis (PG0352) affects the regulation of small RNA associated with inflammation in epithelial cells after infection with porphyromonas gingivalis W83 and its sialidase gene mutant. Methods: anaerobic culture of P. gingivalis W83 and its sialidase gene mutant. (PG0352). The expression of HSA-miR-9, hsa-miR-23aanhsa-miR-23b and hsa-miR-410 in PG0352 group was detected by co-culture array with epi4 of immortalized gingival epithelial cells. Results: the expression of epi4 in PG0352 group was higher than that in P.gingivalis W83 group. Conclusion: compared with P. gingivalis W83 wild strain, 4.33870.4.7930 and 4.3137.Conclusion: compared with P.gingivalis W83 wild strain, Sialidase gene deficient strain has different regulation on the expression of small RNA associated with inflammation in gingival epithelial cells.
【作者单位】：中国医科大学附属口腔医院牙周病科辽宁省口腔医学研究所牙周病学研究室辽宁省口腔疾病转化医学研究中心;中国医科大学附属口腔医院中心实验室辽宁省口腔疾病重点实验室;
【基金】：国家自然科学基金资助项目(编号:81200785) 辽宁省自然科学基金(编号:2015010496-301)
【分类号】：R781.4

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