广西壮族代谢综合征患者血清脂联素水平及其基因多态性分析

发布时间：2018-02-25 03:20

本文关键词： 代谢综合征脂联素基因多态性广西壮族　出处：《山东医药》2017年22期 　论文类型：期刊论文

【摘要】：目的观察广西壮族代谢综合征(MS)患者血清脂联素(APN)水平及其基因多态性。方法将100例广西壮族MS患者作为观察组,100例广西壮族健康者作为对照组,采用酶联免疫吸附法(ELISA)检测两组血清APN,采用聚合酶链反应-限制性片段长度多态性技术观察两组APN基因位点+45 T/G、+276 G/T的多态性。结果观察组、对照组血清APN水平分别为(79.67±7.91)、(104.42±10.67)mg/L,两组相比P0.05。观察组APN+45位点TT、TG、GG基因型分别为16、38、46例,对照组分别为20、59、21例,两组GG基因型比例比较P0.01。观察组ANP+276位点GG、GT、TT基因型分别为61、30、9例,对照组分别为91、8、1例,两组GT、TT基因型比例比较P均0.01。观察组APN+45位点TT、TG、GG基因型患者血清APN水平分别为(81.49±9.42)、(76.17±8.29)、(75.68±8.91)mg/L,TT与TG、GG基因型相比P均0.05。观察组APN+276位点GG、GT、TT基因型患者血清APN水平分别为(80.73±9.58)、(75.49±8.83)、(75.21±8.39)mg/L,GG与GT、TT基因型相比P均0.05。结论广西壮族MS患者血清APN水平低;APN+45 G等位基因、APN+276 T等位基因可能是广西壮族MS患者的易感基因。
[Abstract]:Objective to observe the serum adiponectin (APN) level and its gene polymorphism in patients with metabolic syndrome (MSM) of Zhuang nationality in Guangxi. Methods 100 patients with MS in Guangxi Zhuang nationality were used as the control group and 100 healthy persons in Guangxi Zhuang nationality as the control group. Enzyme linked immunosorbent assay (Elisa) was used to detect serum APNs in two groups. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to observe the polymorphism of 45 T / G, 276 G / T of APN gene in the two groups. The serum APN levels in the control group were 104.42 卤10.67 mg / L, respectively, compared with P0.05.The APN 45 locus TTT GG genotype was 163846 cases in the observation group and the control group in 21 cases. The ratio of GG genotype in the two groups was compared with that in the control group (P 0.01). In the control group, 91 cases were found in the control group, and 1 case in the control group. The serum APN levels of the patients with APN 45 locus were 75.17 卤8.29 mg / L and 75.68 卤8.91 卤8.91 mg / L, respectively. The serum APN levels of the observation group with APN 276 were 80.73 卤9.58 卤8.83 mg / g GG and 75.21 卤8.39 mg / L respectively. The serum APN levels of the observation group were 80.73 卤9.58 mg / g, 75.21 卤8.39 mg / L ~ (-1) and 75.17 卤8.39 mg / g, respectively, compared with those of the control group (P < 0.01). The serum APN levels in the observation group were 80.73 卤9.58 (75.49 卤8.83) mg / g GG and 75.21 卤8.39 mg / L ~ (-1) GG, respectively, compared with those of the control group (P = 0.05). Conclusion the low serum APN level of Zhuang nationality patients with MS in Guangxi may be the susceptible gene of MS patients in Guangxi Zhuang nationality.
【作者单位】：南宁市第二人民医院;
【基金】：广西壮放自治区南宁市科学研究与技术开发计划项目(20123135)
【分类号】：R589

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