甘菊DlNAC1转录因子基因提高烟草耐高温能力

发布时间：2018-02-25 07:19

  本文关键词： 菊花 NAC转录因子 生物信息学 高温胁迫 转基因烟草　出处：《植物研究》2017年03期 　论文类型：期刊论文

【摘要】：研究针对从甘菊中克隆获得的DlNAC1基因(Gen Bank登录号为EF602305)进行生物信息学分析,并利用根癌农杆菌介导的叶盘转化法将该基因在烟草中进行过表达研究。结果发现DlAC1蛋白具有较高亲水性,二级结构中占比最高的为无规则卷曲,并具有N糖基化位点等6类潜在的模体结构和典型的由一个扭曲的反平行β片层和α螺旋组成的NAC结构域。将DlAC1基因在烟草中过表达后,通过PCR方法从55株转化植株中鉴定出36株为阳性植株,并且转基因烟草T_0代植株在45℃高温胁迫后,转35S:DlAC1基因阳性植株生长状况良好,而对照植株发生萎蔫,并且转基因植株叶片含水量显著高于对照植株。然而,在4℃低温胁迫后,发现转基因烟草T1代植株没有提高耐低温能力。甘菊DlAC1基因能够提高烟草植株耐高温能力,为今后菊花抗逆育种提供了科学依据。
[Abstract]:In this study, bioinformatics analysis was carried out on the accession number EF602305 of the DlNAC1 gene Gen Bank, which was cloned from chamomile. The gene was overexpressed in tobacco by Agrobacterium tumefaciens mediated leaf disk transformation. The results showed that DlAC1 protein had high hydrophilicity and the highest secondary structure was irregular curl. There are six kinds of potential motifs such as N-glycosylation sites and a typical NAC domain composed of a twisted anti-parallel 尾 lamellae and a helix. The DlAC1 gene was overexpressed in tobacco. 36 positive plants were identified from 55 transformed plants by PCR method. After 45 鈩，

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