硅对辣椒CaWRKY41基因温度胁迫下表达分析

发布时间：2018-02-26 08:44

本文关键词： 硅辣椒 CaWRKY基因温度胁迫表达分析　出处：《基因组学与应用生物学》2017年02期 　论文类型：期刊论文

【摘要】：WRKY转录因子是植物界中存在的一类较大的基因家族,参与植物在生物和非生物逆境胁迫中的多种应答。为了研究在低温和高温等非生物胁迫对辣椒CaWRKY41基因表达的影响,以豫椒101为实验材料,在对辣椒CaWRKY41基因克隆的基础上,利用生物软件对其编码蛋白进行分析和构建进化树。结果表明,同源扩增获得的序列含有一个990 bp完整ORF框;对其编码蛋白分析发现,只含有一个WRKY结构域,属于WRKY41家族。亚细胞定位在细胞核中,分子进化树表明与茄属亲缘关系最近,相似性达到92%。荧光定量分析表明,与对照相比,低温、高温胁迫都能诱导CaWRKY41基因表达,表明CaWRKY41基因在应对低温和高温胁迫方面具有重要作用。
[Abstract]:WRKY transcription factors are a large family of genes existing in the botanical world, which are involved in many responses of plants to abiotic and biological stresses. In order to study the effects of abiotic stresses such as low temperature and high temperature on the expression of CaWRKY41 gene in capsicum capsicum. On the basis of cloning the CaWRKY41 gene of Yujiao 101, the encoding protein was analyzed and the phylogenetic tree was constructed by using biological software. The results showed that the sequence obtained by homologous amplification contained a 990bp complete ORF frame. It was found that there was only one WRKY domain, which belonged to the WRKY41 family. The subcells were located in the nucleus. The molecular phylogenetic tree showed that the molecular phylogenetic tree had the closest relationship with the genus Solanum, and the similarity was 92.The fluorescence quantitative analysis showed that, compared with the control, the molecular evolution tree showed that, Both low temperature and high temperature stress can induce CaWRKY41 gene expression, indicating that CaWRKY41 gene plays an important role in coping with low temperature and high temperature stress.
【作者单位】：河南省农业科学院园艺研究所;驻马店市农业科学院;
【基金】：国家大宗蔬菜产业技术体系豫北综合试验站项目(CARS-25-G-27) 农业部黄淮地区(河南)蔬菜科学观测实验站(10205020) 河南省重大科技专项(151100110400)共同资助
【分类号】：Q943.2;S641.3

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