利用基因芯片筛选动脉钙化大鼠差异表达基因

发布时间：2018-02-27 01:00

本文关键词： 基因芯片动脉钙化转化生长因子β 基质金属蛋白酶　出处：《广东医学》2016年02期 　论文类型：期刊论文

【摘要】：目的利用基因芯片技术筛选正常大鼠和动脉钙化大鼠腹主动脉组织中差异表达基因及检测转化生长因子-β(TGF-β)、基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的表达量,以探讨动脉钙化发病的可能机制。方法通过皮下注射大剂量维生素D_3建立大鼠动脉钙化模型,采用Von Kossa染色观察动脉钙化程度,基因芯片技术筛选两组大鼠差异表达基因及检测TGF-β、MMP-2和MMP-9的表达量。结果与对照组相比,模型组差异表达的基因有710条,其中上调基因344条,下调基因366条。模型组TGF-β1、TGF-β3和MMP-2的表达均明显上调,差异具有统计学意义(P0.05),而TGF-β2和MMP-9的表达则无明显差异(P0.05)。结论动脉钙化的形成是多基因共同作用的结果,其中TGF-β、MMP-2和MMP-9对动脉钙化的发生发展起着至关重要的作用,其机制还有待进一步研究。
[Abstract]:Objective to screen differentially expressed genes in abdominal aorta of normal and calcified rats and to detect the expression of transforming growth factor- 尾 (TGF- 尾), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in abdominal aorta of normal and calcified rats. Methods A rat model of arterial calcification was established by subcutaneous injection of high dose vitamin D3, and the degree of arterial calcification was observed by Von Kossa staining. Gene chip technique was used to screen differentially expressed genes and detect the expression of TGF- 尾 -MMP-2 and MMP-9 in two groups of rats. Results compared with the control group, there were 710 differentially expressed genes in the model group, including 344 up-regulated genes. The expression of TGF- 尾 1, TGF- 尾 3 and MMP-2 in the model group was significantly up-regulated (P 0.05), while the expression of TGF- 尾 2 and MMP-9 was not significantly different in the model group. Conclusion the formation of arterial calcification is the result of multigene interaction. Among them, TGF- 尾 -MMP-2 and MMP-9 play an important role in the occurrence and development of arterial calcification. The mechanism of TGF- 尾 -MMP-2 and MMP-9 remains to be further studied.
【作者单位】：广西医科大学第一附属医院血管外科;
【基金】：国家自然科学基金资助项目(编号:81160047)
【分类号】：R543

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