DLC-1和SGCZ基因单核苷酸多态性对惠阳胡须鸡生长性状的影响

发布时间：2018-02-27 02:10

本文关键词： 惠阳胡须鸡肝癌缺失基因-(DLC-) 泽塔-肌聚糖蛋白(SGCZ) 单核苷酸多态性生长性状　出处：《黑龙江畜牧兽医》2016年21期 　论文类型：期刊论文

【摘要】：为了解肝癌缺失基因-1(DLC-1)和泽塔-肌聚糖蛋白(SGCZ)基因单核苷酸多态性对惠阳胡须鸡生长性状的影响,以惠阳胡须鸡作为试验材料,从鸡血液中提取DNA,然后通过PCR-RFLP分析DLC-1和SGCZ基因单核苷酸多态性与生长性状的关系。结果表明:93日龄惠阳胡须鸡的DLC-1基因中GG基因型的体重与AA基因型相比差异不显著(P0.05),但GG基因型个体比AA基因型多16.68 g;93日龄惠阳胡须鸡的SGCZ基因中AG基因型的体重与AA基因型相比差异不显著(P0.05)。DLC-1和SGCZ基因限制酶切片段长度多态性对93日龄惠阳胡须鸡的体重无显著影响;但DLC-1基因中GG基因型、SGCZ基因中AG基因型的93日龄样本平均体重最大,比DLC-1基因中AA基因型和SGCZ基因中AG基因型的平均体重多49.62 g。说明DLC-1基因的优势基因型为GG基因型,DLC-1和SGCZ基因的优势基因型组合为DLC-1基因中GG基因型和SGCZ基因中AG基因型,可作为惠阳胡须鸡早期基因型选择的参考依据。
[Abstract]:To investigate the effect of single nucleotide polymorphisms (SNP) on growth traits of Huiyang bearded chickens, Huiyang mustache chickens were used as experimental materials. DNA was extracted from chicken blood, and the relationship between DLC-1 and SGCZ gene single nucleotide polymorphisms and growth traits was analyzed by PCR-RFLP. The results showed that the weight of GG genotype of DLC-1 gene was not different from that of AA genotype in W93-day-old Huiyang bearded chicken. The weight of AG genotype in the SGCZ gene of GG genotype was 16.68 g / 93 days old compared with AA genotype, but the weight of AG genotype was not significantly different from that of AA genotype. The restriction fragment length polymorphism of P0.05N. DLC-1 and SGCZ gene on 93 days of age was significantly different from that of AA genotype. There was no significant effect on the body weight of Yang-bearded chicken. However, the average body weight of 93-day-old sample of GG genotype in DLC-1 gene and AG genotype of SGCZ gene was the largest. The average body weight of AA genotype in DLC-1 gene and AG genotype in SGCZ gene was 49.62 g, indicating that the dominant genotype of DLC-1 gene was GG genotype DLC-1 and the dominant genotype of SGCZ gene was GG genotype in DLC-1 gene and AG genotype in SGCZ gene. It can be used as a reference for early genotype selection in Huiyang Beard Chicken.
【作者单位】：惠州学院生命科学系;广东金种农牧科技股份有限公司;
【基金】：广东省普通高校特色创新项目(自然科学类)(2014KTSCX178) 惠州学院博士启动基金项目(156020021)
【分类号】：S831.2

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