橡胶树胶孢炭疽菌T-DNA标记基因Lv1初步分析

发布时间：2018-02-27 16:46

本文关键词： 胶孢炭疽菌 T-DNA Lv 功能分析　出处：《热带作物学报》2017年02期 　论文类型：期刊论文

【摘要】：通过对实验室已构建的胶孢炭疽菌T-DNA突变体库中各突变体致病力的测定,获得致病力丧失突变菌株T-900,利用TAIL-PCR克隆标记基因侧翼序列,进行比对和序列分析,获取假设基因Lv1的全序列,采用生物信息学方法进行Lv1基因预测及功能分析,最后通过敲除野生型菌株RC178中的基因Lv1进行功能分析。结果表明,获取的假定基因Lv1全序列共4 450 bp,基因预测显示T-DNA侧翼序列位于预测基因的1 727 bp处,正好处于预测基因的第1个外显子内;并且推测该基因为组蛋白H3基因,含有2个内含子,3个外显子;功能验证结果发现敲除突变体△T-900-16与T-900致病力表现一致,推测Lv1基因与RC178的致病力相关。
[Abstract]:The pathogenicity of T-DNA mutants from T-DNA mutants constructed in laboratory was determined, and the virulence loss mutant T-900 was obtained. The flanking sequence of TAIL-PCR marker gene was used for comparison and sequence analysis. The full sequence of the assumed gene Lv1 was obtained, and the Lv1 gene prediction and function analysis were carried out by bioinformatics method. Finally, the functional analysis was carried out by knockout the gene Lv1 from the wild-type strain RC178. The obtained supposition gene Lv1 sequence consists of 4 450 BP. The predicted T-DNA flanking sequence is located at 1 727 BP of the predicted gene, which is located in the first exon of the predicted gene, and is presumed to be the histone H3 gene. There were 2 introns and 3 exons. The results of functional verification showed that the pathogenicity of the knockout mutant T-900-16 was consistent with that of T-900, suggesting that the Lv1 gene was related to the pathogenicity of RC178.
【作者单位】：中国热带农业科学院环境与植物保护研究所;海南大学环境与植物保护学院;三亚市南繁科学技术研究院;中国热带农业科学院热带生物技术研究所;
【基金】：国家自然科学基金(No.31101408) 国家外专局引智项目(No.20154600004) 公益性行业(农业)科研专项(No.201403075)
【分类号】：S763.7

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