重金属Cd胁迫下三七主要药效成分与其合成关键酶DS和P450基因表达相关性研究
发布时间:2018-02-28 18:50
本文关键词: 镉 三七 关键酶 基因表达 药效成分 出处:《中草药》2016年24期 论文类型:期刊论文
【摘要】:目的研究外源性镉(Cd)对三七主要药效成分合成中关键酶2,3-氧化鲨烯被达玛烯二醇合成酶(DS)和细胞色素P450单加氧酶(P450)基因表达的影响。方法设置9个Cd的质量分数梯度(0.0、0.1、0.3、0.6、1.0、3.0、6.0、10.0、30.0 mg/kg)分别对三七进行胁迫实验,两年后采集成熟三七,通过q RT-PCR检测手段对其根部DS及P450基因进行表达量测定,并以GAPDH基因作为内参基因进行DS及P450基因相对表达量的计算,通过相关性分析计算DS和P450基因表达与三七主要药效成分三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1以及总皂苷的相关性。结果 Cd在低质量分数下能促进DS的表达,高质量分数下抑制DS的表达;随着Cd质量分数的增加,对P450表达的抑制作用更加明显;而DS和P450基因表达与三七主要药效成分三七皂苷R1、人参皂苷Rb1、人参皂苷Rg1以及总皂苷无显著相关性(P0.5)。结论重金属Cd对三七药效成分中关键酶DS和P450基因表达影响不同,对同一种酶,Cd质量分数不同其影响也不同。但主要药效成分并未因此受明显影响。
[Abstract]:Objective to investigate the effects of cadmium (Cd) on the oxidation of shark key enzyme of 2,3- synthesis by the 37 major components in graphene dammarenediol synthase (DS) and cytochrome P450 monooxygenase (P450) gene expression of the mass fraction gradient method set 9 Cd (0.0,0.1,0.3,0.6,1.0,3.0,6.0,10.0,30.0 mg/kg) respectively for 37 stress experiment 37, two years after the acquisition of the root mature, DS and P450 gene expression was detected by means of Q, RT-PCR, and GAPDH gene as a control gene DS and P450 gene of the relative amount of calculation, through correlation analysis, calculation of DS and P450 gene expression and 37 of the 37 major components of ginsenoside R1, ginsenoside Rb1 correlation, ginsenoside Rg1 and total saponins. The results of Cd in low concentration could promote the expression of DS and high concentration inhibited the expression of DS; with the increase of mass fraction of Cd, The more obvious inhibitory effect on P450 expression; DS and P450 gene expression and 37 of the 37 major components of ginsenoside R1, ginsenoside Rb1, ginsenoside Rg1 and no significant correlation between total saponins (P0.5). Conclusion Cd on the 37 components of heavy metals in key enzymes DS and P450 gene expression in different effects on the same enzyme the mass fraction of Cd, its influence is also different. But different major components did not significantly affected.
【作者单位】: 北京师范大学中药资源保护与利用北京市重点实验室;宁夏医科大学基础医学院医用化学系;北京师范大学资源药物教育部工程研究中心;
【分类号】:S567.236
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本文编号:1548520
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