烟草PMT基因单核苷酸多态性与烟碱含量变异的研究

发布时间：2018-03-01 03:30

本文关键词： 烟草烟碱单核苷酸多态性　出处：《分子植物育种》2017年07期 　论文类型：期刊论文

【摘要】：为了解析不同烟草品种间烟碱含量差异的遗传机制,深入探索烟碱表型变异与基因序列多样性之间的关系。本研究从29份烤烟品种全基因组重测序所揭示的基因组序列变异中,分析了普通烟草种4个PMT基因家族基因的SNP和Indel变异,筛选错义突变位点。发现在NtPMT2基因第1个外显子的第75个碱基处存在1个错义SNP位点C23_49502707,在供试群体存在G和T两种等位变异。结合8个环境点的烟碱表型鉴定,可知在显著水平上T基因型等位变异供试品种的烟碱平均含量比G基因型的高14.7%,是高烟碱优异等位变异。进而对该位点进行了PCR标记转化,并筛选到1对扩增稳定、带型清晰的功能标记。本研究为克隆烟碱含量调控相关基因奠定了良好的实验基础,为高烟碱品种的分子标记辅助选择提供了可用的标记和基因资源。
[Abstract]:In order to elucidate the genetic mechanism of nicotine content differences among different tobacco varieties and to explore the relationship between nicotine phenotypic variation and gene sequence diversity, this study was carried out in 29 flue-cured tobacco varieties revealed by genome re-sequencing. The SNP and Indel mutations of four PMT gene family genes in common tobacco species were analyzed. A missense SNP locus C2349502707 was found at the 75th base of exon 1 of NtPMT2 gene, and there were two allelic variations G and T in the tested population. The results showed that the average content of nicotine in T genotype allelic variation was 14.7% higher than that in G genotype, and it was highly nicotinic excellent allelic variation. The PCR marker transformation of this locus was carried out, and a stable amplification pair was screened out. This study laid a good experimental foundation for cloning the genes related to the regulation of nicotine content and provided useful markers and gene resources for molecular marker-assisted selection of high-nicotine varieties.
【作者单位】：中国农业科学院烟草研究所中国农业科学院烟草遗传改良与生物技术重点开放实验室;中国农业科学院研究生院;苍溪县扶贫互助社管理办公室;
【基金】：中国农业科学院科技创新工程(ASTIP-TRIC01);中国农业科学院烟草研究所青年科学基金(2015A01)共同资助国家烟草专卖局烟草基因组计划重大专项(110201301008)
【分类号】：S572

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