基于全基因组关联分析研究的2型糖尿病局部基因网络构建初探

发布时间：2018-03-02 08:09

本文关键词： 糖尿病型基因网络生物通路　出处：《中国慢性病预防与控制》2016年02期 　论文类型：期刊论文

【摘要】：目的基于全基因组关联分析(GWAS)的研究结果,初步建立一个2型糖尿病易感基因的局部基因网络图,为今后的基因-基因交互作用,基因-环境交互作用的研究提供参考。方法该研究的65个候选基因选自经过GWAS研究获得统计学关联的2型糖尿病易感基因,从中选取具有2个或以上通路的目标基因27个,并通过通路的筛选、中间基因的选取,构建局部基因网络,并利用Ai CS6软件绘制网络图。网络构建由以下步骤构成:(1)通过KEGG、Genecards数据库获取基因和通路的基本信息;(2)建立初级基因网络;(3)选取中间基因;(4)构建基因二级连接;(5)构建局部基因网络并绘制网络图。结果获得了1个包括27个易感基因和10个中间基因以及一系列连接基因和中间分子的局部基因网络图,该网络包含20条生物通路。结论该研究所得的局部基因网络图较为直观和综合地展示β细胞功能减退和胰岛素抵抗易感基因之间的相互关系,可对深入研究2型糖尿病分子病因机制提供参考。
[Abstract]:Objective to establish a local gene network map of susceptibility genes of type 2 diabetes mellitus based on the results of GWASs. Methods Sixty-five candidate genes in this study were selected from a statistically associated type 2 diabetes susceptibility gene obtained from the GWAS study, and 27 target genes with 2 or more pathways were selected from the study. And through the selection of the pathway and the selection of the intermediate gene, the local gene network is constructed. Using Ai CS6 software to draw the network map. The network construction consists of the following steps: 1) obtaining basic information of genes and pathways through the KEGGG gene bases. (2) setting up a primary gene network / 3) selecting the intermediate gene 4) constructing a secondary gene link (5). A local gene network consisting of 27 susceptible genes and 10 intermediate genes and a series of linked genes and intermediate molecules were obtained. The network consists of 20 biological pathways. Conclusion the local gene network obtained by this study shows the relationship between 尾 -cell dysfunction and insulin resistance susceptibility genes directly and synthetically. It can provide reference for further study on molecular etiology of type 2 diabetes.
【作者单位】：北京大学公共卫生学院流行病与卫生统计学系;
【基金】：国家自然科学基金资助项目(81172768)
【分类号】：R587.1

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