新疆细毛羊eIF3l基因的克

发布时间：2018-03-02 12:18

  本文选题：新疆细毛羊　切入点：eIFl基因　出处：《畜牧与兽医》2017年08期 　论文类型：期刊论文

【摘要】：为了探究新疆细毛羊真核生物翻译起始因子(eukaryotic translation initiation factors,eIFs)基因eIF3l序列和原核表达蛋白特征,采用RTPCR法从新疆细毛羊成纤维细胞中克隆eIF3l基因mRNA的CDS区编码序列,构建其原核表达载体,并进行原核表达和蛋白检测。结果显示:完整获得eIF3l基因1 695 bp,共编码564个氨基酸残基;37℃,1 mmol/L异丙基硫代半乳糖苷(IPTG)诱导3.5 h可形成大量包涵体蛋白,其分子量约为69 ku;Western blot鉴定目的蛋白表达正确。结果表明:新疆细毛羊eIF3l基因原核表达成功,为进一步研究其功能和应用提供了科学数据。
[Abstract]:In order to investigate the eIF3l sequence of eukaryotic translation initiation factors (IFS) gene and prokaryotic expression protein in Xinjiang fine wool sheep, the CDS coding sequence of eIF3l gene mRNA was cloned from fibroblasts of Xinjiang fine wool sheep by RTPCR method. The prokaryotic expression vector was constructed, and the prokaryotic expression and protein detection were performed. The results showed that 1 695 BP of eIF3l gene encoding 564 amino acid residues at 37 鈩，

本文编号：1556492