secA1基因用于诺卡菌菌种鉴定分型的研究

发布时间：2018-03-03 01:25

本文选题：诺卡菌　切入点：secA基因　出处：《疾病监测》2016年12期 　论文类型：期刊论文

【摘要】：目的研究secA1基因在诺卡菌菌种的鉴定分型能力。方法对59株诺卡菌的secA1基因进行聚合酶链反应扩增,扩增片段纯化后测序,并从Gen Bank数据库下载32株诺卡菌secA1基因序列进行补充,采用DNAStar软件对所有序列计算种内与种间相似度水平,并用Mega 6.06软件的邻接法构建诺卡菌菌种系统发育进化树。结果 91株受试诺卡菌secA1基因的种内相似度为97.2%~100.0%,平均相似度达98.6%;种间相似度为85.2%~98.4%,平均相似度达91.8%。以secA1基因构建的系统发育进化树中,16种诺卡菌被准确分离为16个独立的分支,诺卡菌近缘种新星诺卡菌复合体、少食/短链诺卡菌复合体均可有效分离。结论 secA1基因序列分析为诺卡菌菌种的鉴定分型提供了一种新方法,在诺卡菌近缘种的分型方面有独特的优势。
[Abstract]:Objective to study the ability of identification and typing of secA1 gene in the strains of Nocardia. Methods the secA1 gene of 59 strains of Nocardia was amplified by polymerase chain reaction (PCR), and the amplified fragments were purified and sequenced. The secA1 gene sequences of 32 strains of Nocardial bacillus were downloaded from the Gen Bank database and the similarity level between species and species was calculated by DNAStar software. Mega 6.06 software was used to construct the phylogenetic tree of Nocardia strains. Results the intraspecific similarity of secA1 gene in 91 strains of Nocardia was 97.2100.0.The average similarity was 98.6cm, and the interspecific similarity was 85.2%, the average similarity was 91.8%. Because of the phylogenetic phylogeny tree, 16 Nocardia species were accurately isolated into 16 independent branches. The nova nocariella complex and the oligodiet / short streptococcus complex can be separated effectively. Conclusion the secA1 gene sequence analysis provides a new method for the identification and typing of Nocardia strains. It has a unique advantage in the classification of related species of Nocardia.
【作者单位】：温州医科大学检验医学院检验医学教育部重点实验室;中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室;山西医科大学;
【基金】：国家高技术研究发展计划(863计划)(No.2014AA021404) 公益性卫生行业科研专项(No.201302006)~~
【分类号】：R446.5

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