玉米ZmPP6C基因的克隆及其响应光质和胁迫处理的表达模式分析

发布时间：2018-03-03 03:28

  本文选题：玉米　切入点：丝氨酸/苏氨酸蛋白磷酸酶　出处：《作物学报》2016年02期 　论文类型：期刊论文

【摘要】：丝氨酸/苏氨酸蛋白磷酸酶6亚基(catalytic subunits of Ser/Thr protein phosphatase 6,PP6C)是PP6全酶的催化亚基。在模式植物拟南芥中的研究表明,PP6C参与生长素极性运输、脱落酸信号转导和光信号转导途径介导的开花调控。为了明确玉米丝氨酸/苏氨酸蛋白磷酸酶6亚基(ZmPP6C)的蛋白结构特征与同源蛋白间的进化关系,采用RT-PCR方法克隆了ZmPP6C的全长基因。序列分析表明,ZmPP6C开放阅读框为912个核苷酸,编码303个氨基酸残基,包含PP2A的催化亚基PP2Ac结构域;系统进化树分析表明,PP6C蛋白在进化上较为保守,并且与高粱的PP6C蛋白相似性更高。对玉米自交系B73的ZmPP6C基因进行器官特异性表达分析表明,其表达量在成株期叶片中最高,是根中的7.9倍;ZmPP6C能够响应不同光质处理,且受远红光和红光的影响较大;也能响应长日和短日处理,在长日条件下的光照和黑暗阶段各有一个明显的表达高峰,在短日条件下的光照和黑暗阶段分别有2个和3个表达峰值;同时,ZmPP6C还响应高渗透、盐渍和淹水等胁迫处理,出现明显的上调表达。结果表明,ZmPP6C在玉米光信号转导、开花诱导与胁迫应答中发挥重要作用,其分子与生化机制值得进一步探讨。
[Abstract]:Serine / threonine protein phosphatase subunit 6, catalytic subunits of Ser/Thr protein phosphatase 6PP6C, is the catalytic subunit of PP6 holozyme. Studies in Arabidopsis thaliana, a model plant, have shown that PP6C is involved in polar transport of auxin. Abscisic acid signal transduction and light signal transduction pathway mediated flowering regulation. In order to clarify the relationship between the protein structure characteristics of maize serine / threonine protein phosphatase 6 subunit ZmPP6C and the homologous proteins, The full-length gene of ZmPP6C was cloned by RT-PCR. Sequence analysis showed that the open reading frame of ZmPP6C was 912 nucleotides, encoding 303 amino acid residues and containing the PP2Ac domain of the catalytic subunit of PP2A. Phylogenetic tree analysis showed that PP6C protein was more conserved in evolution and more similar to PP6C protein in sorghum. The organ-specific expression of ZmPP6C gene in maize inbred line B73 was the highest in adult leaves. ZmPP6C in root could respond to different light quality treatments, and was influenced by far red light and red light, and could also respond to long day and short day treatment. At the same time, ZmPP6C was significantly up-regulated under high osmotic, salinized and flooded stress. The results showed that ZmPP6C was transduction in maize light signal transduction. The molecular and biochemical mechanisms of flowering induction and stress response should be further explored.
【作者单位】： 河南农业大学农学院/河南粮食作物协同创新中心;中国农业科学院作物科学研究所;中国农业科学院研究生院;北京市辐射中心;
【基金】：北京市自然科学基金(重点)项目(6151002) 国家转基因生物新品种培育重大专项(2014ZX08010-002) 国家自然科学基金项目(31570268,31170267) 中国农业科学院科技创新工程项目的资助~~
【分类号】：S513
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本文编号：1559369