PP1γ基因DNA甲基化在学习记忆中的作用

发布时间：2018-03-04 04:05

本文选题：蛋白磷酸酶γ　切入点：-aza-cdR　出处：《中国实验动物学报》2017年02期 　论文类型：期刊论文

【摘要】：目的研究蛋白磷酸酶1γ(protein serine/threonine phosphatase,PP1γ)和DNA甲基化在学习记忆中的作用。方法通过DNA甲基化转移酶(DNA methytransferases,DNMTs)抑制剂5-aza-cdR处理小鼠,观察小鼠学习记忆情况及其PP1γ表达变化,通过水迷宫测定小鼠的学习记忆能力,Real-time PCR检测小鼠海马区DNMTs和PP1γmRNA转录水平以及Western-Blot测定PP1γ的蛋白质表达水平。为了进一步探讨5-aza-cdR对小鼠学习记忆的影响是否与细胞增殖和凋亡有关,用5-aza-cdR处理NG108-15神经细胞,流式细胞仪、x Celligence系统和荧光素酶报告基因分别检测5-aza-cdR对细胞增殖、凋亡和PP1γ的转录活性的影响。结果侧脑室注射了5-aza-cdR的小鼠空间学习记忆能力增加,同时小鼠海马区的DNMTs和PP1γ的表达降低;10μmol/L 5-aza-cdR抑制细胞增殖,降低PP1γ的转录活性,但是没有诱导细胞凋亡。结论 5-aza-cdR对PP1γ的表达抑制与小鼠学习记忆有关。
[Abstract]:Objective to study the role of protein phosphatase 1 纬 -protein serine/threonine phosphatase PP1 纬) and DNA methylation in learning and memory. Methods mice were treated with 5-aza-cdR, a DNA methyltransferase (DNA) methyltransferase inhibitor, and the changes of learning and memory and the expression of PP1 纬 were observed. Real-time PCR was used to detect the transcription levels of DNMTs and PP1 纬 mRNA in hippocampus of mice, and the protein expression of PP1 纬 in Western-Blot was measured by water maze. In order to further study the effect of 5-aza-cdR on learning and memory of mice, whether it is related to the cells of mice. Proliferation and apoptosis, NG108-15 neurons were treated with 5-aza-cdR. The effects of 5-aza-cdR on cell proliferation, apoptosis and transcription activity of PP1 纬 were detected by flow cytometry, X Celligence system and luciferase reporter gene. Results the spatial learning and memory ability of mice injected with 5-aza-cdR was increased. At the same time, the expression of DNMTs and PP1 纬 in the hippocampal region of mice decreased 10 渭 mol/L 5-aza-cdR inhibited cell proliferation and decreased the transcriptional activity of PP1 纬, but did not induce apoptosis. Conclusion the inhibition of 5-aza-cdR on the expression of PP1 纬 is related to the learning and memory of mice.
【作者单位】：包头医学院中心实验室生物医学研究中心基础学院包头医学院神经科学研究所;内蒙古自治区低氧转化医学重点实验室;首都医科大学宣武医院低氧适应转化医学北京重点实验室;
【基金】：国家自然科学基金(Nos:81460283,81660307) 内蒙古自然科学基金(No:2014MS0810)
【分类号】：Q42

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