不同倍性鲫鲤MeCP2基因分子克隆及时空表达分析

发布时间：2018-03-04 06:07

本文选题：异源三倍体鲫鲤　切入点：异源四倍体鲫鲤　出处：《水产学报》2016年01期 　论文类型：期刊论文

【摘要】：为了探寻不同倍性鱼生殖育性的表观遗传调控规律,实验基于不同倍性鲫鲤精巢的转录本信息库设计引物克隆了不同倍性鲫鲤的MeCP2基因。序列比对结果发现,在远缘杂交的过程中基因组发生了遗传重组和变异。基于序列公共部分设计引物,通过半定量PCR和实时定量PCR的方法分析了MeCP2基因在不同倍性鱼发育过程中的时空表达特征。结果还发现,MeCP2基因在所有组织中都有表达,但在脑组织中的表达量最高;纵向对比性腺的发育过程,MeCP2基因的表达量会随着性腺发育成熟而下降;横向对比不同倍性鱼的性腺发育,MeCP2基因在三倍体鱼卵巢中的表达量明显高于二倍体和四倍体鱼,但在精巢中的表达量随鱼的倍性增加而增加。研究表明,MeCP2基因的表达与不同倍性鱼的卵巢发育密切相关,这为研究鱼类生殖不育的分子机理及其在水产育种上的应用奠定了坚实的基础。
[Abstract]:In order to explore the rules of epigenetic regulation of reproductive fertility of different ploidy fish, primers were designed to clone the MeCP2 gene of different ploidy crucian carp based on the transcripts of testis of different ploidy crucian carp. Genetic recombination and variation occurred in the genome during distant hybridization. Primers were designed based on the common part of the sequence. The temporal and spatial expression characteristics of MeCP2 gene in different ploidy fish were analyzed by semi-quantitative PCR and real-time quantitative PCR. It was also found that MeCP2 gene was expressed in all tissues, but the highest expression level was found in brain tissue. The expression of MeCP2 gene decreased with the development of gonad, and the expression of MeCP2 gene in the ovary of triploid fish was significantly higher than that of diploid and tetraploid fish, and the expression of MeCP2 gene in the gonadal development of different ploidy fish was higher than that of diploid and tetraploid fish. However, the expression level in spermary increased with the increase of fish ploidy. The results showed that the expression of MeCP2 gene was closely related to ovarian development of different ploidy fish. This laid a solid foundation for studying the molecular mechanism of fish reproductive sterility and its application in aquatic breeding.
【作者单位】：湖南师范大学生命科学学院;
【基金】：中国博士后科学基金(2014M560645,2015T80874) 国家自然科学基金重点项目(31430088);国家自然科学基金重大国际合作项目(31210103918) 湖南省科技计划资助(2014FJ3084,2015RS4031) 湖南省生物发育工程及新产品研发协同创新中心(20134486)~~
【分类号】：S917.4

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