肉鸡肥胖基因WDTC1组织表达特性及油脂类型和水平对WDTC1基因表达的影响

发布时间：2018-03-04 21:34

  本文选题：肉鸡　切入点：油脂　出处：《动物营养学报》2016年11期 　论文类型：期刊论文

【摘要】：肉鸡肥胖基因WDTC1(WD and tetratricopeptide repeats 1)是小鼠adipose基因的同源基因,是调控能量代谢、脂肪沉积和血清甘油三酯水平的关键基因。本试验旨在分析肉鸡WDTC1基因组织表达谱及不同类型和水平的油脂对肉鸡不同组织、不同日龄WDTC1基因表达的影响。随机挑选42日龄商品肉鸡母雏5只,进行WDTC1基因的组织表达谱分析。在此基础上,试验选取240只1日龄科宝肉鸡母雏,随机分为8个组(分别在饲粮中添加2.50%、5.00%的亚麻油、玉米油、芝麻油和猪油),每组6个重复,每个重复5只鸡,分别于21和42日龄时从每个重复随机选取1只肉鸡屠宰取样,通过三因素析因试验设计分析油脂类型、水平和肉鸡组织以及油脂类型、水平和肉鸡日龄对肉鸡WDTC1基因表达的交互效应。结果表明:1)WDTC1基因在肉鸡各个组织中广泛表达,其中在卵巢组织中表达量最高,其次是肝脏、肾脏、脑、肺脏、胸肌、心脏、小肠等组织,在胰腺组织中表达量最低。2)油脂类型对42日龄肉鸡不同组织中WDTC1基因相对表达量的影响极显著(P0.01),且油脂类型与肉鸡组织(P0.01)、油脂类型与油脂水平(P0.05)的交互效应显著;2.50%和5.00%油脂水平时亚麻油组42日龄肉鸡肝脏组织中WDTC1基因相对表达量显著高于腹脂组织(P0.05),且亚麻油组肝脏组织中WDTC1基因的相对表达量显著高于芝麻油组(P0.05);2.50%油脂水平时芝麻油组肉鸡腹脂组织中WDTC1基因相对表达量却显著高于亚麻油组(P0.05);油脂类型和水平对42日龄肉鸡胸肌组织中WDTC1基因相对表达量的影响无显著差异(P0.05)。3)油脂类型对不同日龄肉鸡肝脏组织中WDTC1基因相对表达量的影响极显著(P0.01),且油脂类型与油脂水平的交互效应显著(P0.05);2.50%和5.00%油脂水平时猪油组21日龄肉鸡肝脏组织中WDTC1基因相对表达量显著高于42日龄时(P0.05);2.50%油脂水平时亚麻油组肝脏组织中WDTC1基因相对表达量显著高于猪油组(P0.05),5.00%油脂水平时亚麻油和玉米油组42日龄肉鸡肝脏组织WDTC1基因相对表达量显著高于芝麻油组(P0.05)。由此可见,肉鸡WDTC1基因的表达有时空特异性,优势表达组织为肝脏,WDTC1基因表达量受油脂类型、肉鸡组织、肉鸡日龄、油脂类型与油脂水平以及油脂类型与肉鸡组织的交互影响。
[Abstract]:WDTC1(WD and tetratricopeptide repeats 1) is the homologous gene of mouse adipose gene and regulates energy metabolism. The aim of this study was to analyze the tissue expression profile of WDTC1 gene in broilers and the effects of different types and levels of lipid on different tissues of broilers. Effects of different days of age on the expression of WDTC1 gene. Five commercial broilers of 42 days old were randomly selected to analyze the tissue expression profile of WDTC1 gene. On this basis, 240 1-day-old Kebao broilers were selected. They were randomly divided into 8 groups (adding 2.50% linseed oil, corn oil, sesame oil and lard respectively to the diet, 6 replicates in each group, 5 chickens per replica, and 1 broiler was slaughtered and sampled at 21 and 42 days of age, respectively). The interaction effect of lipid type, level, tissue and fat type of broiler, level and age of broiler on the expression of WDTC1 gene was analyzed by three factor factorial experiment. The results showed that the WDTC1 gene was widely expressed in all tissues of broilers. The highest expression was found in ovarian tissues, followed by liver, kidney, brain, lung, chest muscle, heart, small intestine, and so on. The effect of lipid type on the relative expression of WDTC1 gene in different tissues of 42-day-old broilers was very significant, and the interaction between the type of oil and the tissue of broiler was significant (P0.01), and the interaction between oil type and lipid level was significant (P0.05). The relative expression of WDTC1 gene in 42 day old broiler liver tissue of linseed oil group was significantly higher than that of abdominal fat tissue P0.05, and the relative expression of WDTC1 gene in linseed oil group was significantly higher than that in sesame oil group (2.50%) and sesame oil group (2.50%). The relative expression of WDTC1 gene in abdominal fat tissue of normal sesame oil group was significantly higher than that of linseed oil group, but the effect of oil type and level on the relative expression of WDTC1 gene in breast muscle tissue of 42-day-old broiler chicken had no significant difference (P0.05 路3). The relative expression of WDTC1 gene in liver tissues of different day old broilers was significantly affected by P0.01G, and the interaction between lipid type and lipid level was significant (P0.05 + 2.50%) and the relative expression of WDTC1 gene in liver tissues of 21-day-old broilers of lard group at 5.00% oil level was significant. The relative expression of WDTC1 gene in liver tissue of linseed oil group was significantly higher than that of linseed oil group at 2.50% oil level at 42 days of age, which was significantly higher than that of linseed oil group and corn oil group at 5. 00% oil level and 42 days old broiler liver tissue WDTC1 gene relative table. The amount reached was significantly higher than that in sesame oil group (P 0.05). The expression of WDTC1 gene in broilers was space-time specific, and the dominant expression tissue was the liver WDTC1 gene expression, which was influenced by the type of oil, the tissue of broiler, the age of broiler, the type of oil and the level of oil, and the interaction of lipid type with tissue of broiler.
【作者单位】： 河南农业大学牧医工程学院;河南农业大学国家家养动物种质资源平台;
【基金】：国家自然科学基金(31272434)
【分类号】：S831
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本文编号：1567435