呼和浩特地区汉族和蒙古族人群19个STR基因座法医用遗传多态性

发布时间：2018-03-05 08:05

本文选题：法医物证学　切入点：STR基因座　出处：《内蒙古大学》2017年硕士论文　论文类型：学位论文

【摘要】：本文对呼和浩特地区汉族和蒙古族人群19个短串联重复序列基因座(D2S1338、D3S1358、CSF1P0、D5S818、Penta D、Penta E、D6S1043、D7S820、TH01、D8S1179、D12S391、TPOX、D13S317、D16S539、vWA、D18S51、D19S433、D21S11、FGA)的群体遗传多态性进行了调查。通过调查,为呼和浩特地区汉族和蒙古族人群法医学个人识别、群体遗传学以及亲权鉴定研究提供基础参考数据。采用Chelex-100法对所收集的呼和浩特地区汉族、蒙古族群体样本进行DNA提取,并使用基点认知技术(北京)有限公司所生产的GoldeneyeTM身份检定系统20A扩增试剂盒进行扩增及检测。结果呼和浩特地区汉族群体在19个STR基因座共检出238个等位基因和914种基因型,除D8S1179(P0.05)外,其余18个STR基因座分布均符合Jardy—Weinberg平衡定律(P0.05);呼和浩特地区蒙古族群体在19个STR基因座共检出217个等位基因和763种基因型,其19个STR基因座分布均符合Hardy—Weinberg平衡定律(P0.05)。所调查的呼和浩特地区汉族、蒙古族人群19个STR基因座均具有较好识别能力。通过群体差异性检验,呼和浩特地区汉族与蒙古族群体19个STR基因座的等位基因频率无显著性差异(P0.05)。通过人群聚类分析,本次调查研究的呼和浩特地区汉族和蒙古族两个群体最多来自于两个祖先群体.通过群体基因型结构分析,本次调查研究所采用的GoldenEye 20A荧光标记复合扩增系统19个基因座,针对呼和浩特地区汉族、蒙古族群体检验,检出等位基因数量最多的基因座均依次为PentaE,D18S51,D6S1043,检出杂合度最低的基因座均为TP0X与TH01。
[Abstract]:The genetic polymorphism of 19 short tandem repeats (STR) loci D2S1338D3S1358F1P0P0P0D5S818 Penta DPenta D6S1043D7S820 TH01S1179D12S391TPOXA D13S317D16S539vWAA D18S51P19S19S3D21S11FGA) was investigated. The study of population genetics and paternity identification provided basic reference data. DNA was extracted from the collected samples of Han and Mongolian populations in Hohhot by Chelex-100 method. Results 238 alleles and 914 genotypes were detected in 19 STR loci of the Han population in Hohhot region by using the GoldeneyeTM identity verification system 20A amplification kit produced by the basic Point Cognitive Technology (Beijing) Co., Ltd. Except D8S1179 (P0.05), the distribution of 18 STR loci was in accordance with the Jardy-Weinberg equilibrium law (P0.05), 217 alleles and 763 genotypes were detected in 19 STR loci in the Mongolian population of Hohhot. The distribution of 19 STR loci was in accordance with the Hardy-Weinberg equilibrium law (P0.05). The 19 STR loci of the Han and Mongolian populations in Hohhot region all had good recognition ability. There was no significant difference in allele frequencies of 19 STR loci between Han and Mongolian populations in Hohhot. Two populations of Han nationality and Mongolian nationality in Hohhot region in this study were from two ancestral populations. Based on the analysis of population genotypic structure, 19 loci of GoldenEye 20A fluorescence labeling multiplex amplification system were used in this study. For the Han and Mongolian populations in Hohhot, the most detected alleles were PentaEN D18S51D6S1043, and the lowest heterozygosity were TP0X and TH01.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919.4

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